Study On Mixed Fermentation And Screening, Optimization Of The White Rot Fungi In Degrade Stalks

In this paper,we will filter the existing lignin-degrading bacteria-white rotfungi, filter out the good growth, lignin degradation ability of the strains mixed withcellulose-degrading fungus Trichoderma viride co-fermentation of biodegradablestraw,and fermentation of the mixture of the two optimization of process conditions,finally, evaluate the experiments with two in vitro to the following conclusions:1.Screening of six bacteria, especially Phanerochaete chrysosporium andPleurotus ostreatus has the highest enzyme activity of cellulose and hemicellulase,while Ganoderma production Lip and Mnp are significantly better than the otherstrains, it showa that Ganoderma has a strong lignin degradation.2. Through the analysis of single factor and orthogonal test conditions forenzyme production, to study the effects of different culture conditions on thedegradation of straw lignin and cellulose. Set Ganoderma, Phanerochaetechrysosporium and Trichoderma viride two-step enzyme production conditions ofmixed fermentation degradation conditions: potatoes200g/L, glucose20g/L,peptone5g/L, pH6.0speed130rpm, liquid volume of70mL,32°C culture,fermentation to about10day,the crude enzyme solution activity of12.7U/mL.3. Through the analysis of single factor and orthogonal test conditions,concludethe Optimal conditions are as follows: water content of culture medium60%,temperature30℃, the inoculum concentration0.25g/kg, pH control at5.0, at theoptimization of fermentation conditions, the straw lignin degradation rate of49.34%;the wheat straw lignin degradation rate of37.65%.4. The lignin degradation of Ganoderma, Phanerochaete chrysosporium andcellulose degradation Trichoderma viride do plate antagonism teat, foundTrichoderma viride has strong growth advantage, white rot fungus competitiveinhibition. Therefore, the two bacteria need two-step mixed fermentation degradationof straw. Mixed fermentation by different degradation, After the optimization oftreatment, the lignin degradation rate than before, the degradation rate has greatlyimproved, Ganoderma prior cultured for6days,the access of Trichoderma viride co-culture6-8days, the degradation rate of lignin and cellulose are the highest. strawlignin degradation rate reached49.06%the whert straw lignin degradation rate of50.99%, Phanerochaete chrysosporium prior cultured for6to9,the access ofTrichoderma viride days after co-culture about6to10days, Cellulose and lignindegradation rate reached the highest,straw lignin degradation rate of49.34%, thewheat straw lignin degradation rate of49.96%.5. The optimization of feed samples treated in two level in vitro digestion test invitro analysis, find lignin degradation rate of good bacteria mixed combination ofDM digestibility of wheat straw is generally in the range of50%to60%, straw DMdigestibility of40%to50%, the DM digestibility of wheat straw is better than straw.