Studies On The Alkaloids In Uncaria Sinensis (Oliv.) Havil

Objectives: To study the monoterpenoid indole alkaloids in Uncariasinensis (Oliv.) Havil, and the anti-tumour activities of some of the alkaloidsisolated in vitro.Methods: The powdered whole plants of Uncaria sinensis (Oliv.) Havilwere extracted with95%ethanol. After removal of the solvent, the residuewas extracted with petroleum ether, then the aqueous layer was extracted withchloroform to obtain crude alkaloids. Column chromatograghies on silica gel,Rp-18, Sephadex LH-20were applied for the isolation and purification of thecrude alkaloids fraction. The structures were elucidated by theirphysicochemical properties and spectral data.Result: Thirteen compounds were isolated from the whole plants of U.sinensis. On the basis of their physicochemical properties and spectral data,the structures of these eleven compounds were elucidated, including sixalkaloids: Uncarine E (ZXN-1), Uncarine C (ZXN-3), Isopteropodic acid(ZXN-5), Pteropodic acid (ZXN-6), Mitraphylline (ZXN-7),Rhynchophylline (ZXN-8); and five non-alkaloids: Ursolic acid (ZXN-2),Scopoletin (ZXN-4), Noreugenin (ZXN-9), Vanillic acid (ZXN-10),Quinovic acid (ZXN-11). Meanwhile, compounds ZXN-1, ZXN-3andZXN-5were tested for cytotoxic activity against four human cancer celllines in vitro.Conclusion: Compounds ZXN-9, ZXN-10, ZXN-11were isolated from this plant for the first time, and compounds ZXN-1, ZXN-3and ZXN-5did not show cytotoxicity against HL60、A549、K562and BEL-7402cells.