Study On Extraction,Identification And Acute Toxicity Of Alkaloids From Eupolyphaga Sinensis Walker (Blattaria: Polyphagidae)

Dibiechong (Eupolyphaga sinensis Walker), the popular name is Tubiechong, English named EUPOLYPHAGA SEU STELEOPHAGA (ESS), is one traditional Chinese medicine. ESS has comprehensive pharmacology functions, such as dispersing the blood silt, lessening swollen area and promoting blood circulation, et al. Before the Qin and Han Dynasty, our people have used ESS to provent and treat deases. But, the ESS "taste salty, coldly and micro poisonously", which reported according to the literatures. The ESS toxicity and certain pharmacology activeness possibly originate from the alkaloids which contain, but very exiguous research report about the ESS alkaloids was published. In view of these, this article is about extracts, separation and purification method to the ESS total alkaloids, what is more, the ESS alkaloid category and structure have been conducted the preliminary study, in addition the acute toxicity analysis research of the ESS total alkaloids was conducted, and the inorganic elements in the ESS and its total alkaloids have been determined. The obtained main conclusions are as follows:1. Extracte technology of ESS total alkaloidsShuitiChunchen method (water as solvent) and ChuntiShuichen method (ethanol as solvent) were adopted to extract the ESS total alkaloids, and the results showed extract rate of ShuitiChunchen method is higher than that of ChuntiShuichen. According to the single factor experiment and the orthogonal experiment, it was found that pH value is the most important factor which influences ESS total alkaloid extraction, the others respectively are: pH value > temperature > solvent propertion >extract time, and the best extract condition for ESS total alkaloids is: pH2, materiel/solvent (g/ml) 1:12, 120 min, and 80℃.2. Isolation, purification and identification of ESS total alkaloidsThrough pH value gradient liquid extraction, silica gel column chromatographic analysis, thin layer chromatographic analysis carries on the separation and purification to the ESS total alkaloids, 7 alkaloid components were obtained, then their structures were studied by using ultraviolet scanning (UV), infrared spectrum (IR), gas chromatography mass spectrum (GC-MS) and so on. The results indicated that, in the ESS there are many kinds of alkaloid components, steroid alkaloid 1 kind, the amino acid derivate alkaloid 3 kinds, piperidine alkaloid 2 kinds, other kind of alkaloids 8.3. Analysis of elements in ESS and its total alkaloidsInorganic elements in the ESS and its total alkaloids were determined by utilizing ICP-OES, the result accuracy is reliable. The result showed that, the ESS and its total...