The Preparation Of Self-supported Array Electrodes And Their Application In Non-enzymatic Glucose Detection

Glucose detection is very important in biotechnology, medical diagnosis and foodindustry fields. In recent years, with the sufferer of diabetes increases, blood glucosehas become the most frequently checked substance in analysis. Now electrochemicalglucose sensors are important for the glucose detection，which have the advantage ofsimple, fast, high selectivity and so on. Glucose biosensors can be mainly classifiedinto two categories: glucose oxidase based sensors and non-enzymatic glucosesensors. The enzyme sensors have the advantage of better sensitivity and selectivity,but the enzyme is too easy inactivation to make it lack of stability. Recently, thenon-enzymatic glucose biosensors based on metal/metal oxides have received moreand more attention, which have the advantages of fast response, low sample dosage,high sensitivity, easy crominiaturization, simple operation and so on; but in most ofenzymeless glucose sensor studies, the general preparation of electrodes is casting theas-prepared materials onto the surface of glassy carbon electrode or gold electrode, andthen entrapping them with Nafion to prepare modified electrode. Together with thepretreatment steps, such as polishing, modification and drying, the electrodepreparation process is fussy, time-consuming, and weakly reproducible. Otherwise,electrochemical performances are affected by the electrode modification processesconstantly, and the active materials also suffered from falling off easily from theelectrodes. Therefore, exploring effective and tough electrodes to detect glucoseaccurately is urgent. Preparing self-supported electrode on conductive substrate forenzymeless glucose detection is an attractive strategy, in which, active materials growon conductive matrix directly, providing an accessible means without the pretreatmentand modification of the electrode.(1) Self-supported titanium based hierarchical cobalt oxide array electrode wassuccessfully prepared by electrodeposition method, and its electrochemical property innon-enzymatic glucose examination was researched. The results showed that:non-enzymatic glucose sensor builted by this electrode has better stability, repeatabilityand selectivity. This electrode’s sensitivity is526.8AmM-1cm-2, the linear range is3.000mol L-1~1.109mmol L-1, the detection limit is1.37mol L-1. Contrast ofglucose electrocatalytic oxidation between self-supported titanium based hierarchical cobalt oxide nanosheet array electrode and cobalt oxide nanosheet modified glassycarbon electrode showed that the self-supported array structure has great superiority tothe electrocatalytic oxidation of glucose. The electrode was used to examine theglucose in blood serum; the average value of the results was close to the standard valuein hospital, the recovery value was ideal, indicates that this kind of electrode isfavorable in non-enzyme glucose sensor.(2) Self-supported Si-Ti based Cu nanorod array electrode was successfullyprepared by electrodeposition method, and its electrochemical property innon-enzymatic glucose examination was also researched. The results showed that:non-enzymatic glucose sensor builted by this electrode has better stability, repeatabilityand selectivity. This electrode’s sensitivity is1067.5AmM-1cm-2, the linear range is2.000mol L-1～2.156mmol L-1, the detection limit is1.851mol L-1. This electrodewas used to examine the glucose in blood serum; the average value of the results wasclose to the standard value in hospital, the recovery value was ideal; indicates that thiskind of electrode is favorable in non-enzymatic glucose sensor.