Preparation And Properties Of α-Cyclodextrin Polyrotaxanes Loading Sliver Sulfadiazine

Microbes such as bacteria and fungi exist universally in the nature. Although most ofthem are beneficial to human beings and animals and plants, some of them even essential, asmall proportion of them will pose a threat and disease to human health. In order to improvehuman health, and living environment, it has become an important study topic and attracted alot of attention in the past years to explore and develop anti-bacterium materials withbroad-spectrum, high efficiency, no drug resistance and biological safety. Due to some notableadvantages such as broad-spectrum, long-lasting, high efficient and low toxin ect, thesilver-based antimicrobial agents have become a preferred field in antibacterial research.In this paper, we synthesized three series of polyrotaxane (PR1t、PR2tand PR3t) using adifferent molecular weight PEG as chain axis,-cyclodextrin as cyclic small molecules andamantadine as capping agent. The structure of polyrotaxane was characterized by infraredspectroscopy, X-ray diffraction and1H-NMR. The results indicated that polyethylene glycoland-cyclodextrin formed inclusion complexation by non-covalent bond; and polyrotaxanewas scucessfully capped by amantadine. The mainly synthesis factors of polyrotaxane are thereaction time and the molecular weight of polyethylene glycol (MPEG). The synthesis processwas optimized.The antibacterial materials-polyrotaxanes containing silver sulfadiazine (PR-(SD-Ag))were prepared with two series of polyrotaxane (PR1tand PR2t) and silver sulfadiazine bydesigning different mass ratio of polyrotaxane (PR) and silver sulfadiazine (SD-Ag). Thestructure of PR-(SD-Ag) was characterized by infrared spectroscopy, X-ray diffraction and1H-NMR. The light stability of PR-(SD-Ag) and SD-Ag was compared under fluorescent light.The loading capacity and encapsulate efficiency of PR-(SD-Ag) and the Silver sulfadiazine invitro release were tested by UV spectrophotometer. The results indicated that it was not amixture between silver sulfadiazine and polyrotaxane, but the inclusion or assembly wasoccured by hydrogen bonding or hydrophobic interactions of the cyclodextrins, thus silversulfadiazine is clamped by adjacent cyclodextrin. The light stability of PR-(SD-Ag) is betterthan SD-Ag. When the mass ratio of polyrotaxane and silver sulfadiazine is1:1, the loadingcapacity and the encapsulation efficiency are more than90%, this time is the best quality ratio. Silver sulfadiazine was released stably and slowly within6d in vitro, and the release rate ofSilver sulfadiazine rate was over85%.The antibacterial properties of polyrotaxanes loading sliver sulfadiazine were studiedby the oscillation method. The results showed that polyrotaxanes loading sliver sulfadiazinehad excellent antibacterial properties on escherichia coli and staphylococcus, the inhibitionrate was over98%.