A Step Further Analysis Of Cross-kingdom Regulation Of MiRNA-168a In Human Cells

Plant microRNAs(miRNAs) cross-kingdom regulation in animal cells was first discovered in 2011, plants-derived miRNA-168 a can be absorbed through the gastrointestinal tract into the blood circulation and appeard in different tissues in the body. In mammalian liver cells miRNA-168 a targeted regulate the expression of low-density lipoprotein receptor adapter protein 1(LDLRAP1) and thus affecting the body’s metabolism of lipids. This finding confirmed that exogenous food miRNA could enter the body and play a physiological role via cross-kingdom regulation.However, phenomenons of plant-derived mi RNAs regulate human gene expression only have been confirmed in two targets, and two corresponding plant miRNA.Limited references and lag miRNAs cross-kingdom target prediction tools are main reasons for slow development in this field. This paper summarizes miRNAs cross-kingdom regulation experimennts in a most detailed way, and the existing principles of miRNA target predictions were systematically analyzed. First of all,based on our research and according to the limited cross-kindom miRNA targets prediction tools, we summarized how to tap the potential targets of plant miRNA-168 a in human cells from the existing data in NCBI, we added 18 new targets of miRNA-168 a in human body, and our research provide a useful reference for further study of cross-kindom regulation of miRNAs. Secondly, further data analysis revealed that miR-168 a has a potential regulatory targets in human cells,targets slow type of skeletal muscle myosin binding protein C(MYPBC1), this adaptor protein plays an important role in energy supply of skeletal muscle contraction, we designed GFP reporter gene bearing miRNA-168 a : MYBPC1 interacting site and control experiments, after double transfection of miRNA-168 a and recombined GFP reportor gene via lipo-2000 in DU145 cells, and after 24 h the GFP flurescence intensity has a downward effect compared with control group, and subsequent protein immunoblot experiments also demonstrated the negative regulation of mi RNA168 a on MYPBC1 in A549 cells. Therefore, our study initiallyidentified another miRNA-168 a target in human cells. Finaly, we analyised cancer cell proliferation via CCK-8 reagent in different cells transfected with different concentration of miR-168 a, the results showed regular changes while its intrinsic regulation mechanism needs further study.