| Aging is a ubiquitous physiological phenomenon in the organism kingdom.lt is known that aging severely affect female fertility, for example, elevating the risk of spontaneous abortion, chromosomal defects in the offspring, intrauterine growth restriction and preterm delivery. The study in mouse demonstrated that older mothers have shorter lived offspring compared to younger female; Researchof birds in a natural population showed that parental age have a negative effect on its offspring and this effect is sex-specific. Caenorhabditis elegans is the perfect model to research aging, but so far, in the study of C.elegans, just confirmed from the phenotype that parental age has impact on its offspring, had not find the exact molecular mechanism which caused this effect. In this study, we performed with young adult worms (1 day P) and their offspring (1 day F1),aging worms (6 day P) and their offspring (6 day F1) as research object to study the influence of parental age on lifespan and heat shock response in C.elegans. From the phenotype we found that parental age really have a negativeimpact on their offspring about lifespan and HSR. The lifespan of 6 day Fl shortened about 20% compared with 1 day F1, HSR of 6 day F1 declined markedly compared to 1 day Fl. But, this effect disappeared in Fl to F2. We investigated that the level of histone H3K36me3 sharply dropped in 6 day F1 compared to 1 day Fl.This change of protein may be attributed to the declined level of met-1 mRNA. We supposed that met-1 as a target gene may regulated by miRNAs. We performed RNA-seq to compare miRNAs expression between 1day P and 6 day P,1 day F1 and 6 day F1.The results showed that let-7-Sp expression increased in 6 day P, however, it is no differentiation in F1. This might result from way of miRNAs founctions and the effect caused by parental age delivered to next generation via eggs. Our research provided an example that phenotype from parents can deliver to offspring through epigenetic mechanism. |
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