Gene Expression Regulation Of Two Long Non-Coding RNAs During Myoblast Differentiation

In recent years, a lot of studies have focused on the molecular mechanism and function of long noncoding RNA. Based on the previous chip results,we identified and screened several differentially expressed lncRNA and their target genes by tissues expression pattern and correlation analysis. The main results are as follow:1. We identified 19 lncRNAs and 226 lncRNAs remarkable correlated with the marker genes myodl and sgcg respectively with the correlation coefficient of above 0.9 according to the previous studies. The tissue expression profile of lncRNA three months’ mouse indicated the lncRNA AK003290 and NR₀01592 were only expressed in skeletal muscle, AK052919 was expressed in cardiac muscle and skeletal muscle, and AK004418 and AK004293 were widely expressed in all the tissue with highly expression in muscle.2. We verified the expression of four lncRNAs by real-time PCR and analyzed the correlation between lncRNAs （AK003290, NR₀01592, AK004293, AK052919, AK004418,AK048320） and their target mRNAs. The result of real-time PCR shows consistent and significant correlation with RNA Chip.3. For two lncRNAs （AK003290, AK004418） and one target mRNA（myodl）, effective siRNAs fragment were designed and screened. The results demonstrated that knocking down AK003290 can decrease the gene expression of sgcg、trdn and myodl, and decreasing the expression of AK004418 can inhibited the expression of myh3, myhl4, myh8, myh7 and trdn. The expression of lncRNAs AK003290 and NR₀01592 in turn were suppressed by myodl, which revealed that these two lncRNAs were regulated by target mRNA transcription.The research established the foundation for further studies about the function and mechanism of lncRNA in muscle.