Expression And Analysis Of PXY And Its Homologous Genes In Arabidopsis Thaliana

The procambium and cambium are meristematic tissues from which vascular tissue is derived. Vascular initials differentiate into phloem towards the outside of the stem and xylem towards the inside. A small peptide derived from CLE41 is thought to promote cell divisions in vascular meristems by signalling through the PXY receptor kinase. TDIF-PXY pathway plays an important role during the development of vasc-ular; Our results analysis the expression of PXY and homologous gene in Arabidopsis thaliana; we identified a large number of genes specifically regulate the development of vascular tissues.Arabidopsis thaliana PXY, PXL3 and PXL4 encode CLV1-related LRR-RLK that appear to have redundant or overlapping functions. During the research about PXL3、PXL4 overexpression lines, we found that PXL genes have effect on the differentiation of vascular tissues; PXL inhibition the formation of xylem cell. Although PXY and PXL3-. PXL4 belong to the same subfamily, the PXL3and PXL4 overexpression will not rescue or aggravate the pxy.There are many of genes expressed in vascular tissue of Arabidopsis thaliana, they decide the fate of meristematic cell. ATHB-8、WOX4、WOX14 are connected with differentiation of meristem. ATHB-8 can promote procambial cell proliferation and regulate the cells’differentiation into xylem vessel cells; TDIF promoted the expre-ssion of ATHB-8. Our results indicate that the expression of ATHB-8 rely on PXY and PXL3、PXL4; there are antagonism between PXY and PXL3、PXL4. WOX4 and WOX14 is reported to be downstream of PXY, they promote procambial cell prolife-ration. Hirakawa demonstrating that pxy phenotype is not a result of changes in WOX4 expression. We suggest that the expression of WOX4 rely on PXL3 and PXL4; but WOX14 is different.