Single Cell And Single Nanoparticle Analysis With Surface Plasmon Resonance

In the field of life Analytical Chemistry, the common analytical methods include electrochemical analysis, spectrum analysis and nuclear mass spectrum analysis. During the spectrum analysis, molecular fluorescence analysis is the most widely used in the field of life Analytical Chemistry. However, it needs to label the object of study. It is not only waste time, but also interfere the object of study inevitably. On the contrary, a new type of spectrum analysis method developed in recent years, SPR, has the advantages of good signal stability and label-free. In recent years, with the development of SPR, SPR is widely used in the application of single cell and single nanoparticle analysis. We carried out some work in the application of single cell and single nanoparticle analysis:We carry out the study of cell apoptosis in single cell. Cell apoptosis is one of the life science research hot spots, apoptosis detection methods are also emerge in endlessly. There are many methods of treatment of cancer. One of the effective methods is to induce the cell to be apoptotic cells. We urgently need a non-invasive method to test the effects of the new drugs in clinical medicine. Therefore, the research of cell apoptosis is quite important for the application of clinic. During the process of cell apoptosis, PS will overturn. With this advantage, we used SPR to real-time monitor the binding of Annexin V with PS in the apoptosis cells during our research. We can achieve the aim of the detection of cell apoptosis.We carry out the study of determination of irregular gold nanoparticles’absolute concentration. Molar concentration of gold nanoparticles is one of the most critical parameters of gold colloids in order to develop their application in sensing, diagnostics and medicine. Previous methods often stand just for gold nanoparticles with regular shape and narrow size distribution. In the present work, we proposed an absolute quantification method that determined the molar concentration of gold nanoparticles with arbitrary shapes and poly-disperse sizes. This approach involved the real-time monitoring and counting of individual nanoparticles collision events, from which the quantification of molar concentration was achieved using a theoretical model consisting of Fick’s laws of diffusion and Stokes-Einstein equation. The determination of spherical gold nanoparticles concentration resulted in excellent agreement with tradition spectrometry method. This method does not rely on the geometry and optical characteristics of nanoparticles, so it can be applied to determine the concentration of arbitrary shape.