Bioinformatics Analysis Of RBK2 And Its Related Protein Family In Arabidopsis Thaliana And Braasica Napus

A kinase Family that is associated with no extracellular ligand-binding domain(ECLB) and transmembrane domain(TM) are classified as Receptor-like cytoplasmic kinase(RLCK). In plants, RLCK plays key roles in many biological processes, such as signaling transduction, growth, self incompatibility, abiotic and biotic stress responses. The ROP binding protein kinases 2(RBK2) in Arabidopsis thaliana belong to RLCK subfamily VI, it could interact with Rho-related GTPases(ROP),Suggesting that RBK2 contribute important roles in plants. The understanding of RBK2 is not vastly investigated and thus nnovel finding can contribute to the functional aspect of this protein. The allotetraploid Brassica napus is an economically important crop species used for human consumption; a hybrid species within the Brassica genus of Brassica napus is formed between the hybridization of Brassica rapa and Brassica oleracea. Due to the process of Chromosome doubling within this plant no analytical report has been describe in relation to the RBK2 or RLCK proteins.To analyze the structure and function of RBK2 in Arabidopsis thaliana bioinformatic tools were employed. The identification and analysis of RBK2 and RLCK family in Brassica napus was also investigated using the references of the model plant Arabidopis thaliana. The main results are as follows:1. Bioinformatics analysis of RBK2 in Arabidopsis thaliana: RBK2 is a hydrophilic and labile protein, located in the cytoplasm and the nucleus. The secondary structures were mainly composed of alpha helix structure and beta sheet folding. Phylogenetic tree construction and multiple sequence alignment showed that,the RBK2 that evolutionary distance with the related proteins from other speciesis closely related. RBK2 was most widely distributed in the nuclei, and it had a strong interaction with the PP2 C family proteins. The RBK2 gene strongly expressed in the mature pollen had similar expression pattern with many genes associated with polar growth of pollen tubes.2. Bioinformatics analysis of RBK2 in Brassica napus: Using the bioinformaticstools Blast, 4 RBK2 genes were identified in Brassica napus. The protein encoded by BnaA05g32020 D had the same number of amino acids with the protein encoded byBnaC05g46660 D. The same results were observed for the proteins encoded by BnaC03g34140 D and Bna A03g28960 D. However by conducting sequence alignments, protein sequences were not identical as the amino acids. Phylogenetic analysis of four different plants investigating the RBK2 protien found that BnaA03g28960 D came from Bra001138, BnaA05g32020 D came from Bra039458,BnaC05g46660 D came from Bol003162, and there was a RBK2 gene deletion in Brassica oleracea.3. Bioinformatic analysis of Brassica napus RLCK family: 642 RLCK genes were retrieved from the Brassica oleracea genome and further grouped into 19 subfamilies based on their structural and sequence similarities. The structure analysis revealed that, 90% of RLCK genes had the number of exons was less than 10. Protein structure analysis revealed that 77% of the protein sequence contained only intracellular kinase domain. The other structural domains contained in these protein sequences were made up primarily of LRR、TPR、WAK、USP、Ubox、Stress-antifung、Malectin、Lectin、S-locus and PAN. Potiental predication of the functions of the RLCK subfamily in B. napus can be determine fromt the noted results done by this research.