Global Identification And Comparison Of MicroRNA In Wild And Cultivated Adzuki Bean

MicroRNAs (miRNAs) are a class of small regulatory RNAs that function as crucial regulators that repress the expression of their target genes at the transcriptional or post-transcriptional level. Global identification of miRNA is important for the functional study. Adzuki bean (Vigna angularis) genome sequencing was finished recently and lack of a systematical analysis of miRNA. In this study, we performed deep miRNA sequencing in wild (CW108) and cultivated (JN6) adzuki beans. Our investigation not only identified the miRNAs that shared by these two species, but also found miRNAs that specifically existed in individual accession. Through comparison of the miRNA expression and their target between the wild and cultivated adzuki beans, we found that the miRNA has been diverged in these two accessions, which may be associated with plant-pathogen interaction pathway. Our study provided a valuable resource for functional analysis in adzuki bean.