Functional Studies Of Marine Microalgae On Activity Screening And Active Ingredients

In recent years, with the land resources dwindling, humans will sights Marine resources。 Among them, the Marine micro algae because of its special environment, with novel structure, unique functions of active substances, have very good medicinal value, formed the ocean economic growth point.The rise of Marine micro algae industry for micro alga provides abundant raw materials production.This paper with ten common micro algae-Synechocystis sp.PCC6803、Dunaliella salina、 Phaeodactylum tricornutum、Nostoc Flagelliformis、Spirulina、Anabaena sp. PCC 7120、 Nostoc Umbilicarium、Microcystis aeruginosa 905、Scenedesmus quadricanda、 Chlorellavul-garis (Hereinafter referred to as chlorella)> Sporosarcina KluyverVan Niel、 Escherichia coli、Candida Albicans as the research object, the extraction of the active material bacteriostatic experiment, preliminary screening has bacteriostatic action of micro algae.The results showed that spirulina and chlorella exhibit antibacterial effects. Two of the selected microalgae polysaccharides of further research.Spirulina and chlorella powder hot water extraction, centrifugation, the supernatant containing crude polysaccharide solution was initially more impurities. After a series of protein impurities, alcohol precipitation, dialysis, freeze-dried to give a crude solid product polysaccharide. Polysaccharides followed through after DEAE-52 cellulose anion exchange chromatography and Sephadex G-200 gel column chromatography to obtain pure Spirulina polysaccharide. The use of phenol-sulfuric acid assay Spirulina polysaccharide extracted from the sugar content, calculated Spirulina polysaccharide extraction rate of crude polysaccharide and polysaccharide were pure:2% was 83.3%. After UV-Vis spectral scanning, at 260nm and 280nm were not there at the absorption peaks, indicating Spirulina polysaccharide extract does not contain nucleic acid and protein impurities. After scanning Fourier transform infrared spectroscopy to measure its effective role in the group.Rata combination of Chlorella and Spirulina polysaccharide polysaccharide, use antibacterial and antioxidant activity of experiments to explore the proportion of complex polysaccharides. Complex polysaccharides are inhibited E. coli, which Chlorella polysaccharide Quality:Spirulina polysaccharide mass= 1:4, the inhibitory effect is best; chlorella and spirulina polysaccharide complex of Sarcina no inhibition obvious; chlorella and spirulina complex polysaccharides of Candida are inhibited, of which a significant inhibitory effect is Chlorella polysaccharide:Spirulina polysaccharide=1:1 and 1:6 to achieve the best inhibitory effect.Chlorella polysaccharide:Spirulina polysaccharide=1:2, the best performance of the DPPH radical; Chlorella polysaccharide:Spirulina polysaccharide=1:2 and Spirulina polysaccharide:Chlorella= 1:0 against fat best quality peroxidation. Spirulina polysaccharide:Chlorella polysaccharide= 1:2, the best scavenge hydroxyl radicals; Chlorella polysaccharide:Spirulina polysaccharide=1:3, superoxide anion best; Chlorella polysaccharide:Spiral polysaccharide= 1:3, the reducing power of outperforming other groups.