Identificaiton And Characterization Of Multiple Brassinosteroid Weak Mutants And Screening For SEH1 Interaction Proteins

During the research of brassinosteroid(BR) signaling and biosynthetic pathways, genetic mutants have played a very important role. Most of the early identified brassinosteroid signaling and biosynthetic mutants are null mutants, exhibiting extremely dwarfed phenotypes and male sterility, and the null mutants were therefore less useful for genetic characterization of the brassinosteroid (BR)-related pathways. Identification of intermediate signaling mutants such as bril-5 have been contributed significantly to the research of BR signaling pathway using genetic and biochemical approaches. Here we report our identification of several BR mutants of signaling and biosynthetic pathways, and they are the ideal tools for genetic screening. We also provide several evidence to show that we can identify new elements in BR signal and biosynthetic via an activation tagging genetic approach through these mutants. We find a suppressor from the genetic screening of bak1-3 bkk1-1, then proving that it’s a nuclear pore protein (Nup). The nuclear proteins in animals have some researches, however, the study about plants is a little. We choose one of the Nups, SEH1, which is not only expressed in the nucleus, but also in the cytoplasm, to screen using yeast two hybrid. After screening for several times and the confirm experiments, we find two proteins which can interact with SEH1 in the yeast.