| Microgravity environment of space can induce a serial of changes in cells, such as morphology alterations, cytoskeleton disorder and cell cycle disturbance. Our previous study of simulated-microgravity on zebrafish (Danio rerio) embryos demonstrated cep135 might be a microgravity sensitive gene. Quantitative analysis of cep135 was also conducted in zebrafish embryos and zebrafish embryonic cells (ZF4) after 24 and 48 hour microgravity exposure. Beased on these previous study, the distribution and expression pattern of cep135 in zebrafish embryos, as well as the differential expression of cep135 induced by microgravity, were investigated in this study, using in situ hybridization.The results demonstrated that cep135 is expressed evenly at different stages of zebrafish embryos and the expression levels is extremely higher in 24hpf comparing with other stages. Additionally, simulated-microgravity can induce significant increasing of cep135 and the highest increasing level was found in zebrafish embryos after 48-hour exposure to simulated-microgravity.In order to investigate the possible function of cep135 on cell cycle regulation, we first analyized the cell cycle of ZF4 exposed to simulated-microgravity. A significant arrest in G2 phase was detected in ZF4 cell after 24 or 48 hour of simulated-microgravity exposure, respectively. Molecular inspection revealed the underlying mechanism may due to the up-regulation of weel, as well as down-regulation of cyclinBl and cdc2. The results obtained in this study will undoubtedly provide evidence for revealing the function of cep135 under microgravity condition. |
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