The Study Of The Biological Effectsof Electromagnetic Fields On Probiotic Bacillus Sp.

With the gradual exposure of the abuse and side-effects of antibiotics and chemical pesticides, the application of probiotics is more and more attractive in various fields, due to its non-toxic and pollution-free characteristics. However, the study on the influence of electromagnetic field for the probiotics was poor. In this paper, the main research is that the biological effects of probiotic Bacillus sp.under electromagetic field. The electric field intensity increased from 0.5 V/cm to 3V/cm with increments of 0.5V/cm, and the magnetic field intensity increased from 5m T to 30 m T with increments of 5m T. Bacillus sp. was treated with 20 min, 40 min and 60 min under different intensity electromagnetic field respectively, to study on changes of physiology of Bacillus sp., including biomass, p H values, cells membrane penetration of Bacillus sp., the morphology of Bacillus sp., the contents of nutritional components of Bacillus sp. and purification of aquaculture water quality ability of Bacillus sp.. The conclusions are as follows:(1)As a whole, the growth and metabolism level of Bacillus sp. was highest, when it is treated with 40 min under electromagnetic field. Among them, when the electric field intensity was 2.0V/cm and the magnetic field was 15 m T, the density of Bacillus sp. was particularly significant. Therefore, controlling the additional electromagnetic field with the appropriate field intensity and stimulation time can promote the proliferation of Bacillus sp.. The growth and metabolism level of Bacillus sp. was lowest with 60 min treatment, the contents of nutrients and water purification ability were inhibited by the electric and magnetic fields. And the inhibition effect of Bacillus sp. was the most significant when the electric field intensity was 1.0V/cm and the magnetic field intensity was 25 m T. Therefore, it was treated with additional electromagnetic fields for long time, the growth of Bacillus sp. was inhibited clearly.(2) The p H of bacterial liquid was also impacted after treated with suitable electromagnetic field and the optimum time. When stimulation for 40 min, the electric field intensity was 2.0V/cm and the magnetic field intensity was 15 m T, the p H value decreases compared with the control group. The reason maybe that bacterial produced the acidic substances more than the alkaline substance. Therefore, under the suitable stimulation time and the electromagnetic field intensity, the metabolism level of Bacillus sp. was enhanced.(3)From the changes of protein and nucleic acid in supernatant and the micrograph of Bacillus sp.. We can infer that without damaging bacteria, the suitable electromagnetic field changed the micrograph of bacteria cells, enhanced the penetration activity.(4)Suitable electromagnetic field intensity and time, can also increase the concentration of protein and vitamin B6 in the Bacillus sp.. When the time was 40 min, the electric field intensity was 2.0V/cm and the magnetic field intensity was 15 m T, the concentration of protein and vitamin B6 in the Bacillus sp. was the highest.(5)Electromagnetic stimulation can improve the aquaculture wastewater purification ability of the Bacillus sp.. When the electromagnetic field stimulation for 40 min, the electric field intensity was 2.0V/cm and the magnetic field intensity was 15 m T, the water ammonia nitrogen and nitrite nitrogen removal ability of the Bacillus sp. was the most significant.Comprehensive above, We found that it was the most beneficial for the growth and metabolism level of Bacillus sp. bacillus, nutrient content of Bacillus sp., the permeability of Bacillus sp. and water purification ability when the electromagnetic field intensity were 2.0V/cm and 15 m T for 40 min, respectively.