| The East China Sea (ECS), roughly in the range of 21-33° N and 117-131° E, is a marginal sea of the western Pacific, which located on the eastern side of China. ECS is rich in marine biological resources, as well as the resources of oil and gas. Especially, the unique physiological and metabolic characteristics of marine microorganisms play an important ecological role in marine ecosystem. And the systematic classification and identification of them are potential in further research and exploitations, such as enriching the microbial resources and laying the foundation for the research of the functions in biogeochemical cycles and the novel biologically active substance.In this study, three novel bacteria isolated from seawater and sediment of ECS have been investigated using taxonomic analysis by a polyphasic approach, and they are designated as Loktanella sediminum sp. nov., Jiella aquimaris gen. nov., sp. nov. and Aurantibacillus halotolerans gen. nov., sp. nov., respectively.1. Isolation and identification of Loktanella sediminum sp. nov. S3B03T:Strain S3B03T with no flagellum was a Gram-negative short-rod-shaped bacterium isolated from sediment in Okinawa Trough, specially, with PHB granules in cells. The 16S rRNA gene sequences of S3B03 was most closely related to Loktanella litorea KCTC 23883T with 96.16% sequence similarity, and other species of the genus Loktanella with 92.99-95.38%. The DNA G+C contents was 57.6 mol%. Q-10 was the major respiratory quinone. The dominant cellular fatty acids (>10% of total) was summed feature 8 (C18:1ω7c and/or C18:1ω6c). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unknown lipids. On the basis of the polyphasic analysis. S3B031 is considered to represent a novel species in the genus Loklanella, for which the name Loktanella sediminum sp. nov. is proposed. The type strain is S3B03T(=JCM 30120T= DSM 28715T=MCCC 1K00257T).2. Isolation and identification of Jiella aquimaris gen. nov., sp. nov. LZB041T:Strain LZB041T with peritrichous flagellum was a Gram-negative and motile rod-shaped bacterium isolated from offshore surface seawater. The 16S rRNA gene sequences indicated that strain LZB041T most closely related to genera Aurantimonas (96.0-96.4% sequence similarity) and Aureimonas (94.5-96.0%) within the family ’Aurantimonadaceae’. The DNA G+C content of strain LZB041T was 71.3 mol%. Q-10 was the major respiratory quinone. The dominant cellular fatty acids (>10% of total) were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and cyclo-C19:0ω8c. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unknown aminolipid, one unknown phospholipid and one unknown polar lipid. On the basis of polyphasic analysis, strain LZB041T is considered to represent a novel species in a new genus in the family ’Aurantimonadaceae’, for which the name Jiella aquimaris gen. nov., sp. nov. is proposed; the type strain of Jiella aquimaris is LZB04T (= JCM 30119T= MCCC 1K00255T).3. Isolation and identification of Aurantibacillus halotolerans gen. nov., sp. nov. S1203T:Strain S1203T with peritrichous flagellum was a Gram-positive, motile and spore-forming rod-shaped bacterium isolated from sediment in Okinawa Trough. The 16S rRNA gene sequences indicated that strain S1203T formed a lineage within the family Bacillaceae, which was distinct from the most closely related genus Bacillus (86.2-93.8%). The DNA G+C content of strain S1203T was 47.7 mol%. The strain had cell-wall peptidoglycan that was based on acid (meso-Dpm) and unsaturated menaquinone with seven isoprene units (MK-7) as the predominant respiratory quinone. The dominant cellular fatty acids (>10% of total) were anteiso-C15:0. iso-C15:0 and C16:0.The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, a glycolipid and an unknown phospholipid. On the basis of polyphasic analysis, strain S1203T is considered to represent a novel species in a new genus in the family Bacillaceae, for which the name Aurantibacillus halotolerans gen. nov., sp. nov. is proposed; the type strain of Aurantibacillus halotolerans is S1203T (=DSM 28697T= JCM 30067T= MCCC 1K00259T). |
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