Primary Investigation For The Function Of Several Long Non-coding RNA In Mouse

More and more researches have demonstrated that genetic mutation or modification is an important cause of human diseases. Although 93% of the genomes of human are transcribed, only 2% encode for proteins and the remaining 98% is non protein-coding RNAs (ncRNAs), which consists of small ncRNAs and long non-coding RNAs. Existing literatures indicate that lncRNAs play a key role in epigenetic, transcription and post-transcription level and almost all important life events, such as stem cell maintenance, signal transduction, cell proliferation and differentiation, ontogeny, metabolism, and so on. In addation, it would be related to human diseases, such as cancer, respiratory diseases, cardiovascular diseases, neurodegenerative diseases and virus infection. However, the majority of the function of lncRNAs is unclear, hindering the mechanism research for biomedical related to lncRNAs.This paper screened two lncRNAs (AK006720 and AK037838) potential associated with skeletal muscle development and one lncRNA (NONMMUG005147) potential associated with ovary development in mouse after birth by QPCR. We constructed three overexpression vectors, two for skeletal muscle development (CMV promoter), verifying its function and its role related to the key regulatory factors (MyoG、MyoD、Myf5、Myf6 and MEF2c) in C2C12 cell lines; One for ovary development, verifying its function by generating transgenic mouse because of no corresponding cell lines.As a Result, expression levels of two lncRNAs (AK006720 and AK037838) in skeletal muscle after born at day 5 were significant higher (3-4 times) than that after born at day 0 (p< 0.05). Expression level of lncRNA (NONMMUG005147) in adult mouse ovary was significant higher than other tissues (heart, liver, spleen, brain, and skeletal muscle) (p<0.05). Expression level of lncRNA (NONMMUG005147) in mouse ovary after born three weeks was significant higher than in mouse ovary after born 2 days and five weeks (p< 0.05). However, lncRNAs (AK006720 and AK037838) affecting differentiation of C2C12 cell lines was not significant among the key regulatory factors. In addation, we generates three infants, one transgenic mouse with overexpression of ovary specific NONMMUG005147 in consideration of its high expression level in mouse after born at three weeks. But all babies died after born bited by its mother. Wether lncRNA (NONMMUG005147) is associated with ovary development still needs to be futher research in the future.