Regulational Mechanism Of Relate Target Genes Regulated By GmbHLH30 Transcription Factor In Tamba Black Beans Under Al Stress

Aluminum toxicity is one of the main factors that limits the growth of plants in acid solid. Numbers of researchers concerning plant’s response to aluminum stress have identified many response genes that under aluminum stress. In additional to those genes encoding functional protein, the expression of many genes which are encoding transcription factor is induced by aluminum stress. Transcription factor can be combined with cis acting elements in the promoter region of all kinds of stress related genes, activating downstream or the whole systematic genes so as to improve the stress tolerance of plants. Compared with the functional proteins, more response can be produced by transcription factor potentially. Therefore, this study focuses on the role of transcription factor under aluminum stress.Firstly, by using the methods of constructing prokaryotic expression vector, purifying green fluorescent protein encoded by Egfp report gene and preparing polyclonal antibody, the under-tolerance stress expression of transcription factor GmbHLH30 has been identified in the protein level.Secondly, using the methods of chromatin immunoprecipitation, some genes has been screened from Tamba soybean plant under aluminum stress as the downstream target gene of transcription factor GmbHLH30. After three repeating tests, it is found that transcription factor GmbHLH30 under aluminum stress can combined with one fragment in the region of MATE gene promoter, including 3 cis acting elements which are associated with transcription factor bHLH. This combination then has been verified once more in tobacco plant by the construction of specified verification plant vector of transcription activity.In conclusion, transcription and expression of gene encoding GmbHLH30 transcription factor are first induced under aluminum stress. Then the transcription factor is combined with the cis acting element in the region of MATE gene promoter, regulating the expression of MATE functional protein in order to response to aluminum stress and affiliating the damage of aluminum toxicity.