Isolation And Functional Characterization Of Three Transcription Factors Related To Adventitious Shoot Regeneration In Arabidopsis Thaliana

Transcriptional regulation is one of the major regulations in plant adventious shoot regeneration, but the exact mechanism remains unclear. In this paper we used RNA-Seq technology to analyse the different expression TFs between bel-3 which defects in regeneration and the WS wild type. At last we screened and acquired three TFs associated with in vitro shoot regeneration. They respectively take part in the biosynthesis of jasmonate and secondary cell wall, or encode a protein of unknown function. They are all negative regulation factors in adventitious shoot regeneration.We found that At1G74930 affects adventitious regeneration through regulating the biosynthesis of jasmonate; At3G10590, which encoding an unknown protein, we thought it made the callus defective, then affected regeneration; About At5G62380, we found its mutant being sensitive to cytokinin, but the rate of regeneration decreased. So it may have other pathways to regulate regeneration. Although some studies have indicated it participated in the sythesis of secondary cell wall, it need more endeavor to find the relationship between secondary cell wall and regeneration.In this study, we identified three transcription factors associated with adventitious shoot regeneration and conducted thorough research about the function. Not only enriches our knowledge about the transcriptional regulation mechanism of adventitious shoot regeneration, enhancing people’s understanding of transcriptional regulation in regeneration, but also provides valuable information on candidate TF genes associated with adventitious shoot regeneration for future research.