The Mechanisms Induce C4Photosynthetic Pathway In Aquatic Plant Hydrilla

Population growth and the deterioration of the environment is the main reasonleading to the current food shortages. In addition, we focus on the transformation fromC3plant to C4plant. But since the1970s, C4project still have no breakthrough. Wedidn’t have effective results as we expected, including genetic engineering. Thereforeit is an unavoidable problem to explain the mechanisms in C4transformation.Hydrilla is a submerged plant which belongs to Hydrocharitaceae. For adaptingenvironment better it will transform form C3photosynthetic pathway to C4photosynthetic pathway under low-CO2. This conversion appears in originalbackground. While the C4photosynthesis in Hydrilla not depend on the structure ofKranz but single-cell structure instead. This provides a suitable plant material for ourresearches. Therefore the C4photosynthesis mechanisms in Hydrilla may become atheoretical basis for the C4project.In this paper, the design of transformation program Hydrilla photosynthesistransformation, by selecting different time points to draw the materials andtranscriptome analysis, to research the molecular mechanisms of transformation fromC3to C4in Hydrilla. The results of this paper provides some valuable data for thetransformation of a C3plant, concrete results are as follows:Hydrilla C4photosynthesis induced specific programs are cut through intensivetraining and dissolved CO2in the air to create a relatively low concentration of CO2inthe environment, to promote transformation of Hydrilla verticillata. Before conversionand the conversion process we selected time point to draw materials, by comparingthe change in Hydrilla conversion process transcriptome.The results are as follows：（1） Set up the system of Hydrilla culture;（2） Transform Hydrilla from C3to C4-pathway successfully;（3） Measure the δ13C of Hydrilla （4） There are899genes have changed，we also find72transcription factorsrelated to transformation in Hydrilla；（5） Through the KEGG，we found psaD is a down-regulator in PSI，psbA，PsbR， psbU， PsbY are up-regulators in PSII and petC is adown-regulator in cytochrome complex.；（6） We found238genes related to low-CO2respons.（7） We also rely on the FCM to measure the relative genomic content ofHydrilla，which provide us valid data for us to define the evolution statusof Hydrilla and convenience in the depth analysis of the transcriptome.