The Construction Of Myostatin Gene Immune Vector And Its Influence On The Ability Of Muscle Proliferation In Mice

China is a big country of animal husbandry, but China is also the country withlarge population at the same time. What is more, the amount of meat productsoccupation of per person is still very few. And with the increasing of people’s livingstandard, the demand for animal products, especially the high quality of meat productsis more and more high. Therefore, this requires researchers further developedhigh-yield and high-quality meat products. Myostatin (Myostatin, MSTN), also knownas GDF-8, is a member of the TGF-β super-family. And it is a negative regulator ofskeletal muscle growth. It was widespread concerned because of its closely related to"double-muscling trait". The deletion or mutation of Myostatin gene can relieveinhibition of skeletal muscle growth, resulting in muscle hyperplasia and hypertrophy.Therefore, Myostatin gene closely related to livestock carcass traits and meat qualitytraits. Therefore, this experiment for the study of Myostatin gene is necessary.In this study, Myostatin gene coding region was amplified by nested PCR, andthe fragments were ligated into the HBsAg gene3’end in order to increase itsimmunogenicity. It is connected to the eukaryotic expression vector pEF1α-IRES-DsRed-Express2, and then we successfully obtained the fusion expression plasmidpEF1α-DsRed-S-MSTN with Myostatin gene and HBsAg. The fusion plasmid istransfected into bovine fetal myoblasts. And it is indicated that the eukaryoticexpression vector can be efficiently expressed in fetal bovine muscle cells by usingfluorescence quantitative PCR technique and Western blot technique. Further testingis implemented to explore the Myostatin gene on the proliferation of mouse musclewith3weeks-old Kunming mice as experimental object. The experimental resultsshow that, ELISA was used to detect the growth hormone and triglyceride levels inthe serum of immunized mice. The growth hormone in the positive group at9weeks-age is higher than that of control group. And then continue to rise. At the ageof5weeks the triglyceride levels of positive group was significantly higher than thecontrol group (p<0.05), and it has been higher than that of the control group. For bodyweight in mice, the preliminary positive group was lower than the control group, but the weight in positive group was higher than the control group in5weeks-old, thenbecome lower. The growth rate of body weight in the positive group at3weeks-old to5weeks-old have reached to a growth peak, and at3weeks-old to5weeks-old of agegrowth rate of experimental group was significantly higher than that of control group（p<0.05）.In summary, the fusion plasmid pEF1α-DsRed-S-MSTN can successfullyexpressed in bovine myoblasts and mice. The preliminary experimental results showthat the fusion plasmid can produce certain effect on mice of growth hormone andtriglyceride levels and muscle proliferation. This study has laid a foundation forsubsequent experiments and matting for genetic immune bull, the purpose is to furthercontribute to high muscle production of beef cattle breeding, but more complexadjustment still need further study.