Study On Condition Optimization Of Coenzyme Q₁₀ Production By Bacterium And Key Enzyme Genes

Coenzyme Q10(CoQ10) is used as an oral prophylaxis and therapy for cardiovascular and mitochondrial respiratory-chain diseases, among others. This study aims to isolate and optimize the culture conditions of novel bacteria with higher coenzyme Q10-production activity.A novel CoQ10-producing bacterial strain, CA8, was isolated from a soil sample from Ebolowa, Cameroon using a selective medium that contained isoprene as the sole carbon source. Morphological, biochemical and physiological, and phylogenetic analyses of the16s rRNA sequence showed that CA8belonged to genus Proteus, family Enterobacteriaceae. CA8had the closest relationship with Proteus penneri among all reported strains. Thus, it was identified as P. penneri CA8. Under optimized culture conditions, the CoQ10yield of P. penneri CA8reached108.2mg1-1after36h of cultivation in a flask. P. penneri CA8exhibited potential application in the industrial-scale biotechnological production of CoQ10. This report is the first one on CoQ10production by Proteus. The potential industrial applications of CoQ10production by CA8warrant further exploration.Then, two pair of specific primers were designed based on the key enzyme of CoQ10biosynthetic pathway from Agrobacterium tumefaciens BNQ0605and Agrobacterium tumefaciens str. C58, which name were decaprenyl diphosphate synthase encoded by the dps and4-hydroxybenzoate polyprenyltransferase encoded by the ubiA. Two.similar size fragments were successfully cloned from the Agrobacterium tumefaciens AGL1by using genetic engineering techniques. The sequencing results showed that the fragments of sample delivered is as same as the target gene sequence. It was found that the first fragment has93%identity with the dps from Agrobacterium tumefaciens BNQ0605, and the second one has100%identity with the ubiA from Agrobacterium tumefaciens str. C58.