Cloning And Functional Analysis Of SsMBTE Gene In Suaeda Glauce

Drought and salinity are major factors causing the decrease in agriculturalproduction. Cloning of drought and salt-tolerant genes from halophytes will facilitatebreeding of new plant varieties with drought and salt tolerant traits.The goal of this study was to clone SsMBTF from Suaeda salsa.and then toanalyze its function. The results showed that open reading frame of SsMBTF was399bpin length, which had no introns and encoded132amino acids. The putative Mr and pI ofSsMBTF were15544.9Da and10.14, respectively. SsMBTF was a MEF2typeMADS-box transcription factor which was located in the nucleus.Q-PCR analysis showed that SsMBTF could be expressed in root, stem and leaf.Amount of SsMBTF expression was most in leaf and relatively lower in root.Expression of SsMBTF displayed clock rhythm changes which were from low to highand then degressive within24h. SsMBTF was highly expressed by induced PEG.Expression of SsMBTF was slightly affected by other types of stresses-salt, heat, or cold,whereas it was insensitive to ABA, indicating that expression of SsMBTF wasspecifically induced by osmotic stress, and independent of ABA signal pathway.SsMBTF-containing recombinant strain (E.coli BL21) obtained by heterologousexpression grew faster than the control (non-SsMBTF E.coli BL21) under high salt orhigh osmotic stress conditions, suggesting that SsMBTF played a role in protectingrecombinant from growth inhibition exposed to high salt or high drought.The binary vector of SsMBTF overexpression was constructed and transformedinto Agrobaterium to infect Arabidopsis plants. T1gengeration seeds from infectedArabidopsis plants were harvested.