An Investigation On Diversity Of Insect Species And Baculoviruses In Huoditang Forest

In this paper, we researched insect species and soil baculovirus diversity analysis at different altitudes of Huoditang.We extracted DNA from soil by SDS and phenol-chlorofoem. The study first used PCR-DGGE to analyze the community diversity of soil baculovirus of different altitudes. We also analyzed the similarities and differences of its community among those samples; then we analyzed species correlation between insect and baculovirus, following were the major conclusions:1. Investigations of insect species of different elevations in Huoditang forest, middle altitude(37.6)>high altitude(36.2)>low altitude(28.7).2. In the high altitude, the baculovirus species in the soil samples detected was 23 with an average of 17.5. The average of Shannon- Wiener index was 2.4444. Evenness was 0.8586. By comparing the similarity of community, the highest similarity between QH5 and QH7 was 93.6%. The lowest between QH2 and QH6 was 27.9%. The similarity of two samples’ comparison results was more than 60%, which was 41.8% of the total.3. In the middle altitude, the baculovirus species in the soil samples detected was 30 with an average of 19.889. The average of Shannon- Wiener index was 2.376. Evenness was 0.797. When comparing the community similarity, the highest one- QM3 and QM4 was 82%. The lowest one- QM7 and QM9 was 49.8%. The similarity of two samples’ comparison results was more than 60%, which was 95.6% of the total. The similarity was very high.4. In the low altitude, the baculovirus species in the soil samples detected was 29 with an average of 20.8. The average of Shannon- Wiener index was 2.6329.The average of evenness was 0.8704.By comparing the similarity of community, the similarity of two samples’ comparison results was more than 60%, which was 80% of the total. The similarity was high except QL2.