BdAGL6 Genes Overexpressed In Rice

As we all known,Wheat,Rice and Maize are three major food crops in the world, and they are closely related to human life. Flower development can directly affect the formation of seeds, so that it can regulate the crop yields. Brachypodium is a new model plant and it owns many excellent growth characteristics. Brachypodium has small stature, rapid life cycle, simsple growths, and more importantly, its genome is relatively small,which has the smallest grass genome. So based on the above features, more and more scientists to study Brachypodium as the ideal plant in recent years. AGL6 gene family is widespread in plants, and they have an important role on flower organ development. In this paper, we cloned the Bd AGL6 gene and then transformated this gene to rice through the plant transgenic technology, so that we could studied the situation of rice growth. From those steps, we could infer the function of Bd AGL6 gene for Brachypodium and Rice development,at the same time, we could link Brachypodium,Rice and Wheat effectively, and we could also lay the theoretical foundation for improving crop yields.The research results are shown below:1. We discovered that Bd AGL6 gene has three transcripts, and they are sequentially designated as Bd AGL6-1, Bd AGL6-2, Bd AGL6-3. Furthermore, this gene has some alternative splicing sites;2. Bd AGL6 gene has different expressin levels in various organs, which is mainly expressed in flower at high level. So we could infer that Bd AGL6 gene plays an important regulatory role on flower development;3. The three transcripts have different expression pattern and level. We discovered that the expression level of Bd AGL6-3 is significantly higher than the other two transcripts in floral organs. It indicated that Bd AGL6-3,which has the deletion of 21 bases, plays a major role in the regulation of flower development. However, the molecular mechanism is not very clear;4. By transgenic technology, the three transcripts,which were overexpressed, were transferred to rice plant. Then the transgenic plants were named BDO1 and BDO3. But the mortality phenomenon occurred after the Bd AGL6-2,which was overexpressed, transformed into rice callus, unfortunately its specific reason was not clear;5. We observed that the BDO3 transgenic plants would bloom earlier than the normal plants, but the flowering time of BDO1 transgenic plants were similar to the wild-type. Howerer, both transgenic plants had normal flower structure.Heterologous expression is also an excellent means to study genes. Rice and Brachypodium have a common ancestor, and rice transgenic technology is very mature. So we studied Bd AGL6 gene in rice by transgenic technology would be more effective. In conclusion, this research was to study the function of Bd AGL6 gene in rice through plant transgenic technology. We observed this gene has three transcripts, and their expression patterns are quite different.But similarly, this gene and its transcripts all express in flower at a high level. The BDO1 transgenic plants, which Bd AGL6-1 transcript was overexpressed, is normal compared with wild-type plants. Unfortunately, the rice callus were dead after Bd AGL6-2 overexpressed. And the BDO3 transgenic plants,which Bd AGL6-3 transcript was overexpressed, would bloom earlier than wild-type and BDO1 plants. But the flower structure of those two transgenic plants was.No matter what, we can infer that the three transcripts hasve different biological functions. However, the molecular mechanisms of these three transcripts is not very clear.