Functional Analysis Of AGL6 And AGL13 Genes Of Arabidopsis Thaliana.

The study of flower development is not only the hotspot of the core of plant developmental biology, but also the foundation of the developoment biology, that improves the production of crops and qualities. ABCDE model illustrated the molecular mechanisms of floral development as general principles. AGL6 and AGL13 belong to AP1/AGL9 group in MIKC subfamily in MADS-box genes, which functions are redundancy, they are the sister group of the SEP-group. Studies have shown that the function of AGL6 and AGL13 regulate the flowering time, and regulate the floral organ development. But they were not in ABCDE model and other models which illustrated the molecular mechanisms of floral development. Absent of suitable mutant, the functions and mechanisms of AGL6 and AGL13 were cleary not illustrated in Arabidopsis. The study on AGL6 and AGL13, has important theoretical value in aspects of flower development mechanism and plant system evolution, could help or guiding the plant breeding work. This paper we focus on constructing the overexpress or antisense expression vector, and obtained agl6 agl13 double mutant and 35S::AGL6 transgenic plants. Based on the formation of the genetic transformation plant, and the expression differences analysis. The results offers phenotype evidence for understanding the function AGL6 and AGL13 in flower development, and provides new material for further studies on molecular mechanism. The main results are as follows: the first, 35S::AGL6 transformation plant. Its rosette leaves completely transform into carpeloid structures. The single overexpression AGL6 gene could make the leaves ransform into carpeloid structures, similar to the AG and SEP overexpression, which explain AGL6 gene profits to promotion of capel. The second, AGL6 and AGL13 genes are involved in determination of floral meristem identity. Two or three flowers are produced at the position of a flower in the plants of anti-sense expression of both AGL6 and AGL13, exhibiting they take part in determination of floral meristem identity. In addition, AGL6 can activate the expressions of floral meristem identity genes, AP1, SEP2 and SEP3 in leaves. The third, AGL6 and AGL13 are involved in determination of floral organ identity within 4 wholes.The floral organs reduce and lost in the 4 wholes in flowers of anti-sense expression of both genes plants, which supports they have a function in floral organ determination. The fourth, AGL6 and AGL13 direct organ morphologenesis and positioning. Mergements between bract-inflorescence stem, between peduncle- inflorescence stem, amonge sepals and abnomal position of bract and floral organs were examed in the plants of anti-sense expression of both genes. The fifth, AGL6 can activate the expressions of not only floral meristem identity genes, AP1, AP3, PI, SEP2 and SEP3, but also ovule identity genes SHP1, SHP2 and STK in leaves. This resuls provide a window to view their function mechanism.Expresses of AGL6 and AGL13 refine to distal domains of vary organ merastems, which differs to that of other floral organ identity genes. In order to understanding the expression character, a vetor horbor a strcture of AGL13 promotor driving fluorescence protein gene GFP was constructed in this study.