Nonylphenol Algal Bioaccumulation And The Effects To Its Predator

Nonylphenol (NP) was classified as environmental endocrine disrupter. Itsprecursors nonylphenol ethoxylates are widely use in industrial, commercial,agriculture and household applications. Its widespread use has led to the wideexistence of NP in various environmental matrices, such as water, sediment and air. Ithas threatened to the ecosystem and the human beings. This paper focused on thepotential impacts of nonylphenol on freshwater alga Scenedesmus obliquus andmarine alga Isochrysis.sp. Bioaccumulation of nonylphenol in the two kinds of algaewas studied in order to better understand transportation of nonylphenol into the foodchains. And also we study the effects of dietary exposure of nonylphenol to the oysterCrassostrea rivularis and cladoceran Dophnia magna.The research demonstrated that(1)2and4mg/L NP significantly reduced the growth, chlorophyll a, maximalphotochemical efficiency of PSII (Fv/Fm) and the potential activity of PSII (Fv/F0) ofS. obliquus, Meanwhile the growth, chlorophyll a, Fv/Fm and Fv/Fo of Isochrysis.spwas extremely decreased in1and2mg/L of NP.(2) two kinds of algae have a highaccumulation of NP, most accumulated intracellularly. S. obliquus have the ability toaccumulate805μg/g in2hours, the bioconcentration factor is227. The Isochrysis.spaccumulate417μg/g NP in the exposed time of12hours, the bioconcentration factoris742.(3) Vitellogenin(Vg)-like protein levels were determined in digestive gland bythe alkali-labile phosphate(ALP) assay on oyster Crassostrea rivularis, the maleswhich fed with NP treated Isochrysis.sp show a significant increases in ALP (p<0.05)level. At the mean time, the oyster which fed with NP treated Isochrysis.sp show asignificant inhibition (p<0.05) of superoxide dismutase (SOD) compare to the control which fed with NP-free algae.(4) The cladoceran Dophnia magna which fed with NP-rich S. obliquus show an increase on the number of first brood of neonates and thetotal neonates per female. And the day for the first production put off in thetreatments. The average number of neonates per brood have a trend of increase in thetreatments during the expose time. By the mean of LC-MS/MS, determined171.62ng/g NP in the group which fed with S. obliquus that had expose to NP for2hoursand175.57ng/g in the one fed with12hours’ expose to NP.