Genetic Cloning And Functional Implications Of An Acetylcholinesterase2from Aphelenchoides Besseyi

Aphelenchoides besseyi, which is polyphagous and causes damage to Ficus elastica, Hibiscus syriacus, Saintpaulia ionantha. Moreover, A. besseyi also causes damage to Oryza sativa, Zea mays, Fragaria grandiflora, prevention and cure is very difficult. Acetylcholinesterase be able to degrade the acetylcholine, terminate neurotransmitter function of the postsynaptic membrane excitement of stimulation, to ensure the normal neural signal transmission. It plays a key role in the biological nerve conduction. In biological control, acetylcholineesterase gene has close relationship with pest resistance.We are in order to develop A. besseyi biological control agents of high efficiency and low toxicity. A A. besseyi Ab-ACE-2gene, Ab-ACE-2, were found and well studied. Ab-ACE-2is distributed pharyngeal nerve ring, anal nerve ring and nerve trunk of A. besseyi by in situ hybridization. Through the botanical pesticide screening, according to join the latent solvent soluble and insecticidal effect, select the solubility and insecticidal effect of matrine is better. By RNAi silence, first, RT-PCR detect that the gene has been silent; second,phenotypic observation of RNAi nematodes is the same to normal nematodes; again, pesticide dispose RNAi nematodes and normal nematodes, RNAi nematodes survival rate of pesticides is lower,24h,48h and144h significant differences in survival rate,72h,96h,120h,168h and192h extremely significant difference,216h no significant difference, normal nematodes and RNAi nematodes by water is not significant difference in survival rate; Finally confirmed nematodes of the gene silence had no effect on fertility. It can improve insecticidal effect of matrine after gene slienting of Ab-ACE-2. But RNAi technology is difficult to apply production practice of prevening A. besseyi. In that study, we raise acetylcholinesterase inhibitors as adjuvant for improving insecticidal effect of matrine. These data support that Ab-xbp-1is a potential target for developing potent antinematodal drugs against A. besseyi.