| Plant oil is stored as triacylglycerol (TAG), it is a major source of essential polyunsaturated fatty acids for human. Besides, it is an important industrial materials and potential renewal biomass as well. Therefore, improving oil contents of oil crops is the main object for breeders. Nowadays, molecular breeding is one of the most important approaches to improve oil content. And a better understanding of oil regulation mechanism and its biosynthesis in developing seeds is highly necessary. As an important regulated factor in peanut, over-expression of MGAT (Monoacylglycerol acyltransferase) gene in yeast caused an increase in triacylglycerol accumulation. In this research, we cloned its homolog genes LYSOPL2in Arabidopsis and BnCC5in Brassica napus. Then, we analyzed their expressions and functions. Our findings can contribute to increasing oil content and modifying fatty acid composition in oil crops through genetic engineering. The main results are as follows:1. Using homologous gene cloning method, we cloned LYSOPL2in Arabidopsis and BnCC5in Brassica napus.2. Constructed over-expression vector of LYSOPL2and transformed it into Arabidopsis, Constructed RNAi and two over-expression (with double35S promoter and with its own promoter) vectors of BnCC5in Brassica napus.3. Compared the oil content among transgenic lines, T-DNA insertion mutants and wild-type in T3, T4and T5generations through over-expression of LYSOPL2in Arabidopsis. Hence, we found that oil content in transgenic lines was significantly increased (approximately2.6%) compared with wild-type in all the three generations. Besides, there was significant decrease (4.51%) in mutants when compared with the wild type in T3generation. However, mutants in T4and T5generations had no significant difference in oil contents with only0.6%decrease.4. Transformed BnCC5over-expression and RNAi vectors into Brassica napus Westar and J572and obtained transformed plants. |
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