Effect Of The Mechanical Stress On HUVEC And MCF-7Cell Adhesion

To investigate the changes in cell adhesion of HUVEC and MCF-7undermicrogravity and mechanical stress. HUVEC and MCF-7cell were stimulated bygravity and mechanical stress. Microgravity group was used to treat the2D-RWVShorizontal axis of rotation. Mechanical load group use the2D-RWVS vertical axisrotation. FACS, immunofluorescence technique and Western Blot technique was usedto deteat the express situation of Intergrinβ1, E-cadherin and Paxillin in HUVEC andMCF-7cell under two kinds of mechanical conditions. To analyze the relativefluorescence intensity by software. Western Blot technique was used to detect theexpression quantity of Intergrinβ1, E-cadherin and Paxillin in HUVEC and MCF-7cellunder two kinds of mechanical conditions. Using software to perform gray intensityanalysis on experiment results of three types of adhesion protein.The research indicated that, compared with ground group, the express quantity ofadhesion protein under the simulate microgravity group was less than the quantity ofground group. The express quantity decreased gradually in following24h,48h and72h.Under mechanical load situation. But express quantity of adhesion protein under thesimulate microgravity group is more than the quantity of ground group. The expressquantity increased gradually in following24h,48h and72h. The change of expressquantity was not obviously before fist24h. It becomes obvious from the48h. Itobtains the trend of change of cell adhesion after changing mechanics throughcytometry. The result was same with the result of protein relative fluorescence intensityafter immunofluorescence experiment. In conclusion, the cell adhesion will decrease inthe condition of simulate microgravity. The cell adhesion will increase in the conditionof mechanical load.