Plasma Membrane-cell Wall Adhesion Is Involved In Mediating Plant Cell Response To Environmental Mechanical Stress

Intact plant can avoid the harmness induced by adverse or unordinary environment through adjusting their growth rate, changing morphology, producing tropisms and that's what is called the movement of plant. Then, whether plant single cell in vivo can move in response to loaded mechanical stress? If it were done, what kind of movement will take place? And how mechanical signals transmitted into chemical signals or other kind one, as well as what molecules and instruct will be involved in? After we answering these question, we can achieve an explain about the signal pathway of mechanical stress. It is a new pathway differed from traditional chemical signal pathway. And there is extensive knowledge of this kind of response about animal cell, but limited in plant cell. So, we designed experiments to grasp the response progress and the molecule involved in. Then, we can try to conclude a reasonable signal pathway of mechanical stress in plant cell.The main results are listed below.1. By comparing the angles between long axis of elongated cells and the direction of main stress, it showed that cells tend to divided and elongated apeak to the stress. We stained the cell wall by Calcofluor. We see more intense cell wall fluorescence in loaded cells than that in control cells. And there is no obvious enhance in cell wall fluorescence but serious shrinkage of cytoplasm with intense fluorescence. It proved the perturbation in plasma membrane-cell wall adhesion induced by RGD.2. After treating with GRGDS and loading by mechanical stress, cells display no response to the stress. We analyzed the angle and corresponding cell number. It showed no significant correlation between them. It suggests that the RGD-specified plasma membrane-cell wall adhesion is involved in the response of mechanical stress in plant cell.3. Instead GRDGS by cytochalasins B and colchicines respectively before loading mechanical stress. Comparing the treated group with their control, we can draw a conclusion that cytoskeleton is necessary for plant cell in response to mechanical stress.Thinking about these result together, associate the location of plasmame membrane-cell wall adhesion and cytoskeleton and the related experimental data, we can describe a signal pathway initialed by mechanical stress, then sensed and...