| Phytohormones are natural substances having many important regulatory functions in plant growth and development. The content of phytohormone in a plant is very low, and the hormone is easy to be decomposed. Hence, phytohormone research calls for a rapid, precise and convenient measurement procedure for the hormone assay in plants. Exploring new simple, low-cost phytohormone assay methods utilized the characteristics of biosensor technology has very important significance for phytohormone research and agricultural production. Two electrochemical biosensors were developed for phytohormone determination in this paper, as follows:1,A novel enzyme biosensor to detect IAA was devised. The proposed sensor was based on the oxidation reaction of indole-3-acetic acid oxidase (IAAO) with indole-3-acetic acid (IAA). The various experimental parameters such as the operating pH, temperature, absorption time were systemically investigated and optimized as to improve the analytical performance. And the calibration curve for the determination of IAA was obtained under optimal conditions. The results showed significant linearity R2=0.9948, with the content of IAA in the range of 10μg·mL-1～1mg·mL-1 with a detection limit at about 1μg·mL-1.2,An impedance immunosensor based on O-phenylenediamine/glutaraldehyde modified gold electrode for the determination of phytohormone abscisic acid (ABA) was proposed. The operating pH, absorption time, absorption temperature and concentration of anti-ABA antibody were investigated to optimize analytical performance. The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions. The results showed that the detection limit at about 1 ng·mL-1 in the range of 10ng·ml-1～5000ng·mL-1 ABA. |
PDF Full Text Request