Electrochemical Properties And Application In Biosensor Of Biferrocene Derivatives

Research on ferrocene and its derivatives with particularly biochemicalstructure, which possessing some unique properties in physics and chemistry, forexample, aromaticities, redox properties, higher stabilities and with lower toxicity, hasbeen a hot work in their multi-applications in recent years. Biferrocen and its derivativesare different from ferrocene because the interaction of metals in the biferrocen.Ferrocene and its derivatives play a key role in the fields of electrochemical sensors andmolecular assembly based on their excellent redox activities. Recently, more and moreattentions have been paid on their interaction mechanisms and the potentials as thehybridization indicator of electrochemical DNA sensors. The aim of the present work isto research the immobilization methods of new type water soluble mediator and seekafter the feasibility of compound as catalysed biosensors mediator or as electroactiveintercalator. So that, we can offer based information for the research of life sciences.In chapter 1, recent progress of ferrocene and its derivatives are first described.And then the structure of DNA and the interaction mechanismsof small molecules withDNA are emphasized. At the same time, the used methods of the interaction betweensmall molecules and DNA are also summarized. With these described above as basis,the purposes of this article are suggested.The following three subunits are contained in the research section. In the firstsubunit, the electrochemical properties of three water soluble bisferrocene in thesolution have been investigated by cyclic voltammetry (CV). Three compounds have agood electrochemical reversibility in solution, chemical reaction onto electrode isdiffusion controlled.In the second subunit, Glucose oxidase and the inclusion complex in whichFc₂B(NO₃)₂ is guest and theβ-cyclodextrin polymer obtained by condensationβ-cyclodextrin and glutaric dialdehyde is host are covalently immobilized on glassycarbon electrode to give a glucose biosensor. Because of the formation of inclusioncomplex, Fc₂B(NO₃)a is washed out, the stability and the life time of the biosensors areimproved greatly. The Under optimal conditions, the sensor gave a wide linear responserange of 1～18 mmol/L, the equations of linear regression are 1/i_ss=0.0014×(1/c)+ 0.065 (r=0.989) with a low detection limit of 0.5 mmol/L excellent reproducibilityand stability.In the third subunit, the interaction between the complex of Fc₂B(NO₃)₂ and calfthymus DNA is adsorbed in the Au electrode has been investigated by cyclicvoltammetry (CV). The results showed: The statically attraction and inlay mode existedin the interaction of the water-soluble compound with DNA. Owing to the introductionof hydrophilic groups the activity of the interaction of DNA with the compound higherthan that of the ferroceny Schiff base itself. Study on the effect of ionic intensity (Ⅰ) onthe interaction between complex and DNA, the main interaction of is electrostaticbetween complex and DNA forⅠ＜19mM; whenⅠ＞19mM, the interaction isintercalative.This conclusion indicates Fc₂B(NO₃)₂ is a favorable inlay reagent.