Development Of Imaging SPR Analyzer And Its Micro-array Sensing Chips

Surface plasmon resonance (SPR) bio-sensing is a new technology which can detect reactions between biomolecules. It can be used for label-free, high sensitive, real-time detection and has been widely used in biochemistry. This paper introduces the development and experimental application of a new imaging SPR analyzer and its micro-array sensing chips.This paper introduces the theory of imaging SPR analyzer, discusses and proposes a new way to solve problems resulting from non-uniformity of light source.This paper develops a stable optical detection system, an automatic sample and flow system and a angular control mechanism which can precisely change the incident angle of light source. This paper also develops the PC software with friendly interface to control the imaging SPR analyzer.A series of solutions with various refractive indexes are detected by the imaging SPR analyzer. Results show that response curves have very good linearity and that the imaging SPR analyzer can reach a resolution of 1.9×10^-5 RIU (Refractive Index Unit). Using the imaging SPR analyzer and a 3X5 micro-array chip, this paper successfully detects the rabbit IgG vs. goat-anti-rabbit IgG immunoreaction and human IgG vs. goat-anti-human IgG immunoreaction.In conclusion, this paper develops a new imaging SPR analyzer and micro-array sensing chips which can be used for real-time label-free detection of biomolecules. The results show that with the reference cell in the micro-arrays, noises induced by the bulk refractive index changes and fluctuation of ambient temperature can be eliminated, which can improve the sensitivity of the instrument. The biosensor chip can be regenerated so that the cost per chip can be greatly decreased.