The Third Generation Electrochemical Biosensor Based On The Layered Nanomterial Modified Electrode

Recently, much attention has been devoted to the development of the third generation biosensors which were based on direct electrochemistry of redox enzymes and had a more potential perspective than other two generations of electrochemical biosensor. The direct electrochemistry of redox enzymes can establish a model for the mechanism study of electron exchange among enzymes in biological systems, and provide a foundation for discussing the possible mechanism of the thermodynamics and dynamics of the biomolecule's redox. With the development of nanotechnology, many nanomaterials were used to improve direct electron transfer between the redox enzyme/protein and electrodes. And the layered materials were considered one of the most potential inorganic matrix to immobilize the biomolecules.In this paper, the attractive materials—layered nanomterials were used to immobilize the HRP and GOD. The electron exchange between the enzyme and GC electrodes was greatly enhanced in the layered nanomterials film microenvironment. Base on the direct electrochemistry of oxidase, the third generation biosensor was fabricated. This thesis involves the following subjects.1. A new kind nanomaterial—MnO₂ nanosheet was used as the matrix to immobilize the Horseradish peroxidase (HRP). The HRP immobilized in the MnO₂ nanosheets film maintained its bioactivity. Horseradish peroxidase entrapped in MnO₂ nanosheets films cast on glassy carbon electrode...