| Objective: To investigate the effects of different concentrations of uric acid on the expression of genes related to Wnt/β-catenin signaling pathways during the process of human osteogenic differentiation of h BMSC.Methods: Healthy adult human bone marrow mesenchymal stem cells were in vitro isolated in vitro by adherent culture of whole bone marrow. The Passage 3 h BMSCs were divided into 5 groups: blank control group and the experimenta lgroup(osteogenic induction group) using different concentrations of uric acid(0, 140, 280, 560 μmol/L). The morphology of cells were observed under inverted microscope. After the 7 days of induction, the alkaline phosphatase staining and the alkaline phosphatase activity weredetected. Cell proliferation capacity were detected with CCK-8 on the 3th and 7th day,.Finally, Wnt3α and β-catenin m RNA expression in the Wnt signaling pathways were detected using the method of fluorescence quantitative PCR after the 10 days of induction. The results were analied with statistical methods.Results: Under inverted microscope visible clumps,a large number of adherent cells were observed to form a spindle and they were growing to a typical colonyon the walls. Alkaline phosphatase staining was positive in the experimental group. After treatment with uric acid, alkaline phosphatase activity and cell proliferation capacity were increased, the expression of Wnt signaling pathway-related genes Wnt-3a and β-catenin was up-regulated in a dose-dependent manner. There were significant differences in the abovementioned indices between experimental groups and between each experimental group and control group(P < 0.05).Conclusions: These findings suggest that uric acid up-regulates Wnt-3a/β-catenin signaling pathway and thereby promotes the osteogenic differentiation and proliferation of bone marrow mesenchymal stem cells in a dose-dependent manner. |
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