Molecular Mechanism Of The Regeneration Of Human Gastric Carcinoma Cells By

Gastric cancer is one of the common malignant neoplasms of gastrointestinal tract and a global health problem of common concerns. China is a big country of gastric cancer.Most patients in our country were diagnosed at an advanced stage and missed the opportunity for radical surgery. Chemotherapy is the main therapeutic method in advanced stage gastric cancer but its therapeutic effect is not optimistic and it is accompanied by significant therapy-related toxicity. Traditional Chinese Medicine plays an important role in the treatment of gastric cancer. Previous researches have proven that the XinJiaLiangFuFang is able to inhibit the proliferation of gastric cancer through the induction of apoptosis of gastric cancer cells, regulation of tumor angiogenesis and inhibition of oncogenes expression. This research further clarifies the mechanism of antitumor action of XinJiaLiangFuFang in gastric cancer through the caspase-3,p53 and miR-34a gene related pathway and provides a basis for clinical treatment of gastric cancer.Objectives:1.To observe the inhibitory effect of XinJiaLiangFuFang serum and Dioscin serum on the proliferation of human gastric cancer cells BGC-823.2.To explore the possible mechanism of action of XinJiaLiangFuFang serum and Dioscin serum from the aspect of caspase-3, p53and miR-34a expression level.Methods:1.To prepare XinJiaLiangFuFang serum and Dioscin serum and act on the human gastric cancer cells BGC-823 and detect the cell proliferation inhibitory rate using MTS assay.2.To observe the effect of XinJiaLiangFuFang serum and Dioscin serum on the expression of caspase-3 and p53 in human gastric cancer cells BGC-823 using RT-PCR method.3.To detect the expression level of miR-34a in gastric cancer cell using RT-PCR method.Results1.XinJiaLiangFuFang serum and Dioscin serum have significant cell proliferation inhibitory effect against the human gastric cancer cells BGC-823 and its effect is directly proportional to time. The proliferation inhibitory effect of XinJiaLiangFuFang serum is better than Dioscin serum at 48h and 72h.2.The expression level of caspase-3 in human gastric cancer cells BGC-823 is lower than the normal gastric mucosa epithelial cells GES-1,while its p53 expression level is higher than the normal GES-1 cell. After the XinJiaLiangFuFang serum and Dioscin serum have acted on the BGC-823 cell, its caspase-3 gene expression level significantly increased and the expression level has increased with time,while the p53 gene expression level decreased and is inversely proportional to time.The regulation of caspase-3 and p53 gene between XinJiaLiangFuFang serum and Dioscin serum has no statistical difference,but there is a general tendency that XinJiaLiangFuFang serum is better than Dioscin serum.3.The expression level of miR-34a in gastric cancer cell is higher than the normal gastric mucosa epithelial cells GES-1.Conclusions:XinJiaLiangFuFang and Dioscin can significantly inhibit the proliferation of human gastric cancer cells BGC-823 and their effect may be related to the increase of caspase-3 expression and the inhibition of mutated p53 expression.MiR-34a might play a role of oncogene and it is necessary to study further for the next research.