Fabrication And Reaction Kinetic Of The Biomacromolecular And Its Application For Piezoelectric Biosensor

The modification of biomacromoIecuIars is very important for thedetection. separation and purification of many substances in Iife science. ltis aIso a hot topic in the construction of all kinds of biosensors. How tomodify the biomacromolecufar onto the sensor surface effectively. simpfy.conveniently and quickIy is the first probIem shouId be solved in theconstruction of biosensor. Piezoelectric bulk wave acoustic sensors, knownas very potentiaI sensing devices, have many advantages, such as highsensitivity. low cost. simplicity to use and ease to real-time output etC. Thequartz crystaI microbaIance based on the response of mass change, is asensitive mass sensor and very suitable to the detection ofbiomacromoIecuIar. Now it is expected to find widely use in cIinic chemistry,pharmaceutics anaIysis, environmentaI detection and so on. ln this thesis,diff6rent kinds of marcromoIeculars were modified on to the crystaI surfaceindividually. Their properties on the surface were studied and the resuItswere used as evidence fOr the seIection of sensitive membrane ofmacromolecular. In addition, the theory of fractal reaction kinetics wasappIied to study biomoIecuIar reaCtions on the surf8ce of the piezoeIectricsensor in the first time. FinalIy two kinds of piezoeIectric biosensors wereconstruCted using different sensing membranes and were firstly applied indetecting M. tuberculosis and trichinosis, which have aroused muchattention in recent years. Some more detaiI investigations were listed asfolIowing;1. Protein A and styrene-butadiene-styrene (SBS) were selected assensing membrane to cross Iink antibodies; SBS, poIyvinyI chloride(PVC) and poIystyrene (PS) are polymers and have many advantagessuch as easiIy obtained, convenientIy forming membranes and easi1ymodified by the introduction of other functional groups. PVC. PS andSBS were used as precoating materials for immobiIizing antibody inour experiment. SBS was selected as antibody carrier because it canform a homogenous. stable and effective membrane quickly. ltsmembrane properties and effect on cross-Iinking biomoIecuIars werediscussed. Protein A was selected as a sensing membrane owing tothe faCt that it contains a active spot which can bind Fc portion ofantibodies. So the orientated immobilization of antibody wasachieved. The properties and reusabiIity of the membrane werestudied.2. In this thesis, the fractaI reaction kinetics analysis was first to use inthe study of heterogeneous reactions happened on the surf8ce ofpiezoelectric sensor The adsorption kinetic modeI of antibody andthe immunoreaction kinetic modeI of antibody binding antigen on thesurf8ce of piezoeIectric sensor were set up. When the models wereappIied to the experimentaI, good fitting results were obtained.3. TWo seIected membranes materiaIs were used to f8bricate tWo kindsof piezoe1ectric immunosensors. One was based on the SBSsensitive membrane and "gas phase" immunoassay technology. Byimmobilized anti-TB or anti-trichinosis on the SBS membrane, it canbe used to determine M. tuberculOsiS and trfohiflosjS. The other onewas based on the protein A sensitive membrane and "liquid phase"immunoassay technoIogy. This sensor was applied to the rapiddeteCtion of M. tuberculosiS.