| Purpose:To observe how the blood lactic acid turning point and free radical turning point of different levels of endurance training rats in the incremental load swimming changed, to probe dynamic changes between the blood lactic acid and the free radical as well as the intrinsic association.Research contents:Different levels of endurance training rats under the increasing load swimming to exhaustion,1. The curve of MDA in heart, liver, kidney lung,brain tissue whether exists an obvious inflection point,2. Whether the blood lactate inflection point and heart, liver, kidney, lung, brain, MDA-curve inflection point are postpone in synchronization with the improved level of training,3. Blood lactate inflection point and the heart, liver, kidney, lung, brain, MDA inflection point occurs in the order.Research methods:108 male SD rats were randomly divided into three groups, each denoted by a low tolerance level of T1 group, medium-endurance level of T2 group, high endurance levels of T3 group. Each group included 36 rats, and each group was randomly divided into six sub-groups, each sub-group included 36 rats. The three groups of rats under different intensity of endurance swimming training for 9 weeks, T1 group take no-load swimming training for 40min,60min,80min of each three weeks, T2 group take swimming training burdened with 1% of their body weight for 40min,60min,80min of each three weeks, T3 group take swimming training burdened with 2% of their body weight for 40min,60min, 80min of each three weeks.48 hours after the completion of the training, each training group of the six sub-groups, from 2% body weight load, swimming with the load of 1% of body weight increase respectively, at all levels of the completion of incremental load, each load duration 3 minutes, the last group to swim to exhaustion. Each sub-group to perform a given load swimming then anesthesia and killed. Rats were tested blood lactate and heart, liver, kidney, lung, brain MDA content.Results:1. Blood lactic acid in rats with the increasing load on the rise, T1:The blood lactate of the 6% load sub-group is significantly higher (P<0.01) than the 5% load sub-group, T2:The blood lactate of the 7% load sub-group is significantly higher (P<0.01) than the 6% load sub-group, T3:The blood lactate of the 9% load sub-group is significantly higher (P <0.01) than the 8% load sub-group.2. MDA change:Three groups of rats heart, liver, kidney MDA had roughly the same trend, MDA content were increase with incremental exercise intensity. T1:The MDA content of the 7% load sub-group is significantly higher (P＜0.01) than the 6% load sub-group, T2:The MDA content of the 8% load sub-group is significantly higher (P<0.01) than the 7% load sub-group, T3:The MDA content of the 9% load sub-group is significantly higher (P<0.01) than the 8% load sub-group. Lung and brain MDA had no significant change in trend.3. T1 rats blood lactate inflection point occurs at 6% load, MDA inflection point appears in the 7% load; T2 rats blood lactate inflection point appears in the 7% load, MDA inflection point appears in the 8% load, T3 rats blood lactate inflection point and the MDA inflection point are present in 9% load sub-group.Conclusion:1. Endurance training can reduce the blood lactate concentration and MDA content, Indicating body endurance training can improve the aerobic oxidation capacity and the antioxidant system function.2. In the incremental load swimming to exhaustive exercise, three different training levels rat's blood, heart, liver, kidney, and other organizations MDA contents were increased with exercise intensity increased, and there is a clear inflection point; the lung and brain MDA did not change significantly. The results suggest that lung, brain antioxidant capacity may be greater.3. The blood lactate inflection point and MDA inflection point of different endurance training levels rats were delayed in step, and the blood MDA inflection point fell behind the blood lactate inflection point. The regularity of the dynamic change is significantly.4. The results suggest that in addition to blood lactate inflection point can be used as indicators for monitoring exercise intensity, the blood MDA inflection point can be used as a upper limit index of monitoring exercise intensity.
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