| Purpose: from the overall level, this paper was to explore the law of turning point of blood lactate and MDA's dynamic changes in different training levels of human by treadmill exercise from continuous increasing workload to exhaustion. It also verified the objective exist of "radical threshold" in huaman body, which would lay the foundation for the relationship between dynamic changes on the two turning points and mitochondrial function.Contents: By readmill exercise from continuous increasing workload to exhaustion in different tolerent levels in young men, this paper was, firstly, to observe continuous dynamic changes between MDA in blood and content of blood lactic acid, and to determine whether there was a turing point on blood MDA as an indicator of the dynamic curve. Secondly, it was to determine whether it would post-shift regularly in different tolerent levels'young men if there was a turning point. Thirdly, it was to determine the order and time of turning point in blood lactate and possible blood MDA.Methods: The study objects were divided into two groups, 13 male students with second level athletes of Sports Training in Dept. of Physical Education and Sports Science and 14 male students with non-sports professional but helthy and sports hobby in Hebei Normal University. The 13 male students with high endurance exercise was S Group and the 14 male students with general endurance was G group. The exercise mode was readmill exercise which started from the speed of 6km / h and with the speed of 2km/ h for 3 minutes in each later level for six levels completed by Group S and five levels completed by Group G. The vein blood of elbow was taken at the end of each level. And the MDA and lactic acid were determined by colorimetry.Results: 1. During readmill exercise with continuous increasing workload, there was a an increase on blood lactate with exercise intensity in Group S and Group G.. The exercise time and the workload speed were far higher in Group S than that in Group G. The blood lactate from the second lecel was obviously higher in Group G than that in Group S. And the turning of blood lactate was obviously later in Group S than that in Grou G..2. During readmill exercise with continuous increasing workload, there was obvious difference between Group S and Group G on human's MDA from the first level. However, there was litter difference between the two groups from the second and third levels and the MDA remained stable. MDA began to emerge continuing turning point in Group S and Group G from the fourth level, but the turning point was obviously later in Group S than that in Grou G..3. During continuous increasing workload exercise, the trend on the dynamic changes of MDA and blood lactate was broadly the same. Under the same time with exercise workload, MDA and blood lactate was lower in Group S than that in Group G. The tuning points of MDA and blood lactate were also later in Group S than that in Grou G..Conclusion: 1. Compared with Group G, thus during exercise it reduced generation of blood lactate and MDA which was regarded as an indicator of free radical generation in Group S.2. During readmill exercise with continuous increasing workload, there was turning point on MDA and blood lactate in Group S and Group G. And the dynamic change trends were generally the same which lagged with the raise of endurance level of exercise. The results verified the threshold of free radicals existed in human body, which were consistant with animals experiment.3. During readmill exercise with continuous increasing workload, the time and intensity of turning point of free radical were lagged behind in MDA than that in blood lactate for Group S and Group G. . |
