Effects Of The Resistance Exercise On The Function Of The Mitochondria In Skeletal Muscle Of Aging Rats

Mitochondria is the main position where the free radical generates.85%oxygen that the organism consumes is from electron transport chain.95%of the oxygen free radical is from the electron leak of the mitochondria.ROS of the organism increases with aging,and increasing of the ROS mainly beacase of the electron leak of the mitochondria.Increasing of the ROS leads to the function of the mitochondria,witch causes the obstruction of the cells.Then the function of cells appease exceptional even die and declying of the capacity of stress.So the oxidizing injure of the mitochondria may be the first factor of the function of the aged organism.As a provoking,exercise preasents many actions to the function of the organism.Many reaserches verify that aerobatic exercise may decrease the level of the MDA.Exercise cause the mitchondria coming into suitability changes and promoting the function of the oxidative phosphorylation.There are many researches about the relation between exercise,functiong of mitochondria and aging,but the definite theory is not unknown and the style of exercise is alone relatively,most of them are aerobatic exercise.In this research the modes of aging are got by ingecting D-Galactose.We research the activity of the ETC enzemies and the CS,opening level of the PTP and△ψm.It atampts to investigate the influences of exercise of ladering by loading to the functions og the aging rats,and explaining the meaning and potential application in deferring aging as a new model of resistance exercise.The results:(1)The body weight of the rats increased before and after the researche and there is not observably differences.(2)The contents of the MDA in the quadriceps muscle of thigh of the aging control group is higher than the young control group observably (P＜0.05);The contents of the MDA in the quadriceps muscle of thigh of the young exercise group is lower than the young control group,but it is not observably; The contents of the MDA in the quadriceps muscle of thigh of the aging exercise group is lower than the aging control group,but it is not observably too.(3)The opening of the PTP of the aging control group is higher than the young control group observably(P＜0.05);The opening of the PTP of the young exercise group is lower than the young control group observably(P＜0.05);The opening of the PTP the aging exercise group is lower than the aging control group extremely observably(P＜0.01).(4)The△ψm of the aging control group is lower than the young control group observably(P＜0.05);The△ψm of the young exercise group is higher than the young control group observably(P＜0.05);The△ψm of the aging exercise group is higher than the aging control group observably.(5)The activity of the complexⅠ+IⅢof the aging control group is lower than the young control group extremely observably(P＜0.01);The activity,of the complexⅠ+Ⅲof the young exercise group is higher than the young control group extremely observably(P＜0.01);The activity of the complexⅠ+Ⅲof the aging exercise group is higher than the aging control group extremely observably(P＜0.01).(6)The activity of the complexⅣof the aging control group is lower than the young control group observably(P＜0.05);The activity of the complexⅣof the young exercise group is higher than the young control group,but it is not observably;The activity of the complexⅣof the aging exercise group is higher than the aging control group,but it is not observably too.(7)The rate between the complexⅣand complexⅠ+Ⅲof the aging control group is lower than the young control group observably(P＜0.05);The rate between the complexⅣand complexⅠ+Ⅲof the young exercise group is higher than the young control group observably(P＜0.05);The rate between the complexⅣand complexⅠ+Ⅲof the aging exercise group is higher than the aging control group extremely observably(P＜0.01).(8)The activity of the CS of the aging control group is lower than the young control group extremely observably(P＜0.01);The activity of the CS of the young exercise group is higher than the young control group extremely observably(P＜0.01);The activity of the CS of the aging exercise group is higher than the aging control group observably(P＜0.05).The conclusion: (1),The model of aging designed in this research is succssful.(2),Resistance exercise can decrease the growing rat of body weight,but the fluence of exercise to aging rats is bigger than younger ones.(3),Resistance exercise can inhibite the decreasing of the△ψm,bring down the opening of the PTP.It is use for maintain the balance of the matters in and out of the mitochondria membrance.(4),Resistance exercise can decrease the level of the MDA.(5),Resistance exercise can promote the activity of the complexⅠ+Ⅲ,Ⅳand complexⅠ+Ⅲ/Ⅳ,reduce the products of the free radical and inhibit the decreasing of the functiongs of oxidative phosphorylation in aging rats.(6),Resistance exercise can promote the activity of the CS,witch promote the contents of the mitochondria potein by choicely.(7),Resistance exercise designed in this research is successful.