| Objects:This research aimed at discussing the effects of Hypoxia of different time and training apoptosis of the Renal of Rats,in order to find expression of HIF-1a,EPO,Bcl-2 and Bax of the Renal of rats and the relationships between HIF-1aand related genes of apoptosis.Methods:60 male SD rats were randomly divided into 6 groups: normoxia group(A),hypoxia of 8h group(B),hypoxia of 12h group (C),normoxia training group(D),hypoxia of 8h training group(E), hypoxia of 12h training group(F).The rats of group D,E and F were introduced to treadmill running on an incline of 0 at 25m/min for 1h.After training,the rats of group B and E,C and F were exposed to hypoxic chamber with 12.5%concentrations of oxygen(equal to altitude 4000m) for 8h and 12h every day,five days per week,for 4 weeks.Results:1.The RBC,Hb and HCT were showed:the comparisons between group B and E,and group A and D were significant(P＜0.05).2.The slice was showed in HE dyeing:In the group A and B,the structure of rats' renal cell is integrated and clear,core is big and circular; In the group C and F,renal cell structure changes gradually;In the group D and F,renal cell begin to be obviously swelling and the gap is obviously enlarged between cell,sinusoid is narrowed even vanishes,the core becomes small.3.The TUNEL dyeing slice showed that:between the groups A and D,there was a significant difference(P＜0.05);and between the groups E and F,there was a significant difference(P＜0.05);and between the groups C and F,there was a significant difference(P＜0.05).4.Immunohistochemical technology and computer image processing method were applied to exam the location and content of HIF-1aon renal tissue.The result showed HIF-1aof renal tissue in B and C were significant compared with A;HIF-1a of rnnal tissue in group E and F,A and D were all significant.5.The dyeing slice of Bcl-2,Bax and EPO showed:With the extension of time,male-dyeing grains increased gradually and the grey degree was strengthened.The statistic results showed that Bcl-2 and Bax of renal tissue among A,B and C groups were significant;Bcl-2 and Bax of renal tissue in group E and F,C and F,A and D were all significant.The statistic results showed that EPO of renal tissue among A,B and C groups were not significant;EPO of renal tissue in group E and F,C and F,A and D were not all significant.6.The relations between Renal apoptosis and these items showed that there was a highly significant positive correlation among AI and HIF-1a,Bcl-2 and Bax(P＜0.05).Conclusion:1.The hypoxia could increase the level of RBC,Hb and HCT,the proper hypoxia and training could increase the capability of exercise.2.HIF-1a of renal tissue was increased significantly by intervention with hypoxia,training and hypoxia training.HIF-1a of renal tissue in F was much higher.3.Bcl-2 and Bax of renal tissue was increased significantly by intervention with hypoxia,training and hypoxia training,and Bcl-2 and Bax of renal tissue in F was much higher.But EPO of renal tissue was not increased significantly by intervention with hypoxia,training and hypoxia training.4.Bcl-2 and Bax participated in regulating the renal apoptosis,and the balance relationship between the two were important factors if they would maintain or induce apoptosis.HIF-1a may control and regulate the mechanism of Bcl-2 protein family and other related genes on apoptosis of renal cell. |
