Effects Of Hypoxia Training On The Myocardial Hypoxia Induced Factor-1α And Related Genes Of Apoptosis Of Rats

Objects: This research aimed at discussing the effects of Hypoxiaof different time and training apoptosis of Rats, in order to findexpression of HIF-1α,Bcl-2 and Bax of rats and the relationshipsbetween HIF-1αand related genes of apoptosis.Methods: 60 male SD rats were randomly divided into 6 groups:normoxia group (A), hypoxia of 8h group (B), hypoxia of 12h group (C),normoxia training group(D), hypoxia of 8h training group(E), hypoxiaof 12h training group (F). The rats of group D,E and F were introducedto treadmill running on an incline of 0 at 25m/min for lb. Aftertraining, the rats of group B, E, C and F were exposed to hypoxicchamber with 12.5％concentrations of oxygen (equal to altitude 4000m)for 8h and 12h every day for 4 weeks.Results: (1) The RBC, Hb and HCT were showed: the comparisonsbetween group B and E, and group A and D were significant(P＜0.05).(2) The slice was showed in HE dyeing: In the group A and B, thestructure of rats' myocardial cell is integrated and clear, core isbig and circular; In the group C and F, myocardial cell structurechanges gradually: In the group D and F, myocardial cell begin tobe obviously swelling and the gap is obviously enlarged between cell,sinusoid is narrowed even vanishes, the core becomes small.(3) The TUNEL dyeing slice showed that: between the groups A andD, there was a significant difference(P＜0.05); and between the groupsE and F, there was a significant difference(P＜0.05).(4)Immunohistochemical technology and computer image processingmethod were applied to exam the location and content of HIF-1αoncardiac tissue. The result showed HIF-1αof cardiac tissue in B andC were significant compared with A; HIF-1αof cardiac tissue in groupE and F,A and D were all significant.(5) The dyeing slice of Bcl-2 and Bax showed: With the extension of time, male-dyeing grains increased gradually and the grey degreewas strengthened. The statistic results showed that Bcl-2 and Bax ofcardiac tissue among A, B and C groups were significant; hypoxiainduced factor-1αof cardiac tissue in group E and F, A and D wereall significant.(6) The relations between Myocardial apoptosia and theae itemsshowed that there was a highly significant positive correlation amongAI and HIF-1α, Bcl-2 and Bax(P＜0.05).Conclusion: (1)The hypoxia could increase the level of RBC, Hb andHCT, but the hypoxia and training could decrease the level of RBCHb and HCT.(2) HIF-1α, Bcl-2 and Bax of cardiac tissue was increasedsignificantly by intervention with hypoxia, training and hypoxiatraining. With the hypoxia time longer, HIF-1αof cardiac tissue wasmuch higher.(3) Bcl-2 and Bax participated in regulating the Myocardium'sapoptosis, and the balance relationship between the two were importantfactors if they would maintain or induce apoptosis. HIF-1αmay controland regulate the mechanism of Bcl-2 protein family and other relatedgenes on apoptosis of cardiac cell.