| This thesis introduce a sensitive method for detecting the dopping of Erythropoietin。 Animal were immunized to produce the polyclonal antibody of Erythropoietin, by the labeling of the antibody, develop a simple, rapid, economical method for the in vitro detection of Erythropoietin.Optimizing the substrate and the concentration of enzyme labled antibody, TMB was chosen as the substrare, and establised relatively steady ELISA method antigen and antibody detection.We establised a suitable method for animal immunization by anti-serum prepared, purified and titre detection..and establised a simple method for acquiring high concentration, high purity, high titration polyclonal antibody, the titration of this antibody is about ten times of the polyclonal antibody has reported.Two different method of antibody labled method were compared: the HRP labled antibody method is difficult to control, as the enzyme is easily to form self-cross link, and the non-specific is high in the subsequent detection, however, the biotin labling method has small damnification to antibody, and the non-specificity is low.Use the prepared polyclonal antibody as a capture antibody, and the biotin labled antibody as the detecting antibody and applied different detection system to detect EPO. the result shows that there is good linearity below 10ng,and the sensitivity is lng(1 × 10-8g/ml),it is the nearly the same as the monoclonal antibody, but it is simple and cheaper...
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