Research On Supplementing Bilirubin To Improve Exercising Rats' Ability Of Combating Oxidative Stress

As the studies deepened in recent years, people realized more and more clearly that the bilirubin is not only the simple metabolic productions of heme but also a natural antioxidant in body, which has strong anti-oxidization ability. Many external tests proved that different forms of bilirubin in the physiological density can suppress the damage of every organization caused by free radical effectively.However, rare research on the anti-oxidization ability of bilirubin in sports has been carried untill now. So we try to analyze it's anti-oxidization ability and it's mechanism through measuring the ability of oxidative stress of rat's plasma, membrane of red cell and liver, etc. after the rats were supplemented external source bilirubin. We hope that it can offer experimental basis for the application of bilirubin in the sports fields.The subject in the research were 44 healthy male SD rats, which were divided into different groups at random: control group(C), exhausted group(E), exhausted+ bilirubin group(EB), strong training group(T), strong training + bilirubin group(TB) o The exhausted model and the strong training model were set up respectively in the experiment.The experimental result shows: (D,The total bilirubin of serum of group EB and TB are higher than group E and group T notably separately(P<0.05), and also higher than the control group at the same time, but there is no statistics meaning(P> 0.05).(2),As far as the group EB is concerned, the MDA of plasma and liver less than group E notably, and the MDA of the membrane of red cell is less than group E, too. But there is no remarkable difference(P>0.05);The SOD of plasma, membrane of red cell and liver are higher than group E, and there are remarkable differences in the last two organization(P<0.05 and P<0.01); The CAT and GSH-Px of plasma and liver are higher than group E notably; The GSH-Px of the membrane of red cell are higher than group E (P>0.05) .(3), As far as the group TB is concerned, the MDA of plasma, the membrane of red cell and liver are less than group T(P<0.05); The SOD of plasma and the membrane of red cell are higher than group T notably,and the SOD ofliver is higher than T, but there is no statistics meaning(P>0.05); The CAT of plasma and liver are higher than group T notably; The GSH-Px of plasma, the membrane of red cell and liver are higher than group T, the plasma is shown very remarkable difference(P < 0.01) , the others shown remarkable difference(P < 0.05).(D,The hemoglobin, red cell and Hct of group TB are higher than group T, and the first two indexes shows very remarkable difference(P < 0.01), the last shows remarkable difference(p <0.05).From the above results of our experiment we can draw the following conclusions: (1), The level of bilirubin of serum will declined notably after exhausted moving or strong training, but it will be improved when rats are supplemented external source bilirubin.(2),Supplementing external source bilirubin can improve rat's SOD,CAT and GSH-Px , reduce the production of MDA of every organization notably after exhausted moving or strong training, It suggests: bilirubin is a kind of strong antioxidant. It can improve the lever of anti-oxidization of rats, and it can also reduce the damage of every organization caused by the increased free radical. So it can reinforce the ability of combating oxygen stress of moving rats. (3),The mechanism of bilirubin's function of anti-oxidization may be determined by its characteristic of offering hydrogen; (4), Supplementing external source bilirubin can prevent rats from the dropping of Hb, Hct and red cell caused by strong training. It suggests: supplementing external source bilirubin can slow down the emergence of the exercise anaemia to a certain extent.