Effect Of Malate-oligosaccharide Solution On Antioxidant Capacity Of Endurance Athletes

NADH(coenzyme I ) and NADPH(coenzyme II) is the currency of reduction ability in most organisms, and thus may determine the redox state in vivo. It's the key element of the regulation of energy metabolism, and can provide reduction potential for anabolism.L-malate is an important intermediate in the process of metabolism. Catalysed by malate dehydrogenase(EC 1.1.1.37) and malic enzymes(EC 1.1.1.38, EC1.1.1.39 and EC 1.1.1.40), L-malate may take part in sevral responses to produce NAD(P)H. It plays an important role in tricarboxylic acid cycle and in malate-aspartate shuttle. As an important source of NAD(P)H in vivo, malate might affect the antioxidant ability by changing the redox state.The purpose of this investigation is to observe the effect of malate-oligosaccharide solution on antioxidant ability during aerobic exercise.20 male endurance-trained cyclists were divided into 3 groups: the contrast group(A), the oligosaccharide solution group(G), and the malate-oligosaccharide solution group(M). First, each subject performed incremental cycling to exhaustion to determine the VO2max. After 2 days' resting, each of them performed 45 minutes ergometer exercise at 60% VO2max. Venous blood was taken before and after exercise to assess the concentration of MDA, total antioxidant capacity(TAOC), VC and GSH in serum .RESULTS: (1)The serum MDA of the G group was significant-ly lower than the other2 groups before and after the 45 minutes exercise(p<0.05). (2)The serum TAOC of the G group was significantly higher than the A group before the exercise (p<0.05), and higher than either the A or G groups after the exercise (p<0.05). (3)The serum VC of the G group was significantly higher than the other 2 groups before and after the exercise (p<0.05); the serum GSH of the G group was higher than both the A and G groups after the exercise (p<0.05) . The results indicate that the malate-oligosaccharide solution may increase the antioxidant capacity of athletes in endurance exercise.CONCLUSION: The supplement of malate-oligosaccharide solution may improve the level of antioxidants in vivo after exercise, and subsequently increase the total antioxidant capacity and decrease the level of lipid peroxidation. One possible mechanism may be that malate may increase the level of NAD(P)H in vivo, and subsequently increase the antioxidants and antioxidant capacity.