Effect Of Diazepam In Tissues On The Development Of Chrysomya Megacephala (Diptera: Calliphoridae) In Different Temperature And Implication Of This Effect On Estimation Of Postmortem Intervals Using Arthropod Development Patterns

Objective: To study the effects of diazepam to the development of the larvae of Chrysomya megacephala in different temperature and accumulate the biological data on necrophagous flies in Shijiazhuang area, and to help providing scientific data for deducing the postmortem interval (PMI) in criminal investigation.Methods: Adult Chrysomya megacephala were collected from the outdoors. They were reared successively in a biochemical culture box. The experiment was done with offspring of the third generation. Five domestic rabbits were prepared. The first one was used as a control and the other four were given diazepam via ear vein according to the different dosages of 5mg/kg, 10mg/kg, 15mg/kg and 20mg/kg. All the rabbits were sacrificed by hitting the head quickly 30 minters later. The muscles from the five rabbits were taken down and stored. They were marked with M0, M1, M2, M3 and M4 as the dosages ranged from 0mg/kg to 20mg/kg. The temperatures of the biochemical culture box were fixed to 16℃, 20℃, 24℃,...