Effects Of Psychological Stress On Endocrine Function In Diabetic Rats And Preliminary Study Of Its Mechanism

Objective:Through the establishment of experimental rat model of type 2 diabetes,given psychological stress observed psychological stress its role in endocrine function.Preliminary study of psychological stress through the hypothalamus-pituitary-adrenocortical axis (HPA) and sympathetic-adrenal axis (sympathetic nervous system,SNS) happened two ways to the development of diabetes-induced neural mechanisms for control of diabetes and its course provide evidence-based complications.Methods:Male SD adult rats were given high-sugar high-fat diet with low-dose repeated intraperitoneal injection of streptozotocin (STZ) to make type 2 diabetes rat model.On this basis in rats given psychological stress (forced cold swim and restraint stress), observed the effects of Chronic psychological stress on endocrine function in diabetic rats.65 male SD rats (weight 180±20g) were randomly divided into 4 groups.Separately are:1the control group,2diabetic model group,3 Psychological stress group,4diabetes model + psychological stress group.Observation of general status changes in rats,blood glucose tester Test fasting blood glucose in rats, using enzyme-linked immunosorbent assay(ELISA) detected serum cortisol, epinephrine(EPI),norepinephrine(NE),radioimmunoassay(RIA) detection of serum insulin,glucagon,calculate insulin action index(IAI).SPSS11.5 statistical software used for data analysis.Results:1,Body mass changes:The group 2 and 4 physique in both groups decreased,the group 1 and 3 group in both physical growth.The group 3 was lower than the value of body mass growth in Group 1,and there is significant difference(P<0.05).The group 4 than in Group 2 to reduce the value of body mass,and there is significant difference (P<0.01). 2,Glucose metabolism:The group 2 Fasting plasma glucose increased, IAI lower,compared with the group 1 have significant difference (P<0.01);insulin lower,compared with the group 1 have significant difference (P<0.05);glucagon increased,compared with the group 1 have no significant difference.The group 3 glucose and glucagon compared with the group 1 at the same level as the basic;insulin lower,IAI increased,compared with the group 1 have no significant difference.The group 4 than Group 2 hyperglycemia,there is significant difference (P<0.05);than in Group 2 of the glucagon increased, there is significant difference (P<0.05);than in Group 2 of the insulin-lowering, there is significant difference (P<0.01);than in Group 2 IAI increased,there is no significant difference.3,Neuro-endocrine:The group 2 cortisol,EPI and NE compared with the group 1 at the same level as the basic, no significant difference.The group 3 than Group 1 cortisol rise,there is significant difference (P<0.01);The group 3 than in Group 1 increased EPI and NE,there is no significant difference.The group 4 than in Group 2 cortisol,EPI and NE increased,there is significant difference (P<0.01).Conclusion: Psychological stress affect the level of glucose metabolism in diabetic rats,Caused by a series of endocrine changes in indicators, increase the progression of diabetes;Psychological stress by activating the hypothalamus-pituitary-adrenocortical axis (HPA) and sympathetic nervous system (SNS) the two pathway,resulting in glucose metabolism in rats and endocrine dysfunction; Psychological stress may be the development of diabetes or risk factors predisposing factor.