The Expression Of Notch Signal Pathway And Its Significance In Renal Cell Carcinoma

Objective:Detecting the Notch signaling pathway related protein expression level by immunohistochemistry in human renal cell carcinoma,to explore the possible relationship between the Notch signaling pathway moleculars and the development of human renal cell cancer,providing some direction and theoretical basis for finding new targets in the clinical treatment of renal cell carcinoma.Method:Obtain of 8 pieces the renal cell carcinoma specimens by surgical methods, the Notch signaling receptor Notch-1, Notch-2 and ligand Jagged-1, Jagged-2 protein levels in renal cell tissue and renal cell carcinoma adjacent tissues were detected by HE and immunohistochemical staining methods.The results showed that there are some relevance of the occurrence of renal cell carcinoma and Notch signaling pathways, and that the Notch signaling pathway may be involved in the development of renal cell carcinoma. Results:The positive rate of the memberof Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 was 22.5% , 30%, 65% and 60% , in renal cell carcinoma ,respectively. The positive rate of the member of Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 was 72.5% , 67.5%, 27.5% and 20% , in renal cell carcinoma adjacent tissue,respectively. Compared with renal cell carcinoma adjacent tissue, the positive rate of the memberof Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 have statistical significance in renal cell carcinoma(p﹤0.05).Conclusion:1. It showed that there are some relevance of the Notch signaling pathway protein and the development of human renal cell carcinoma .2. Compared with the renal cell carcinoma adjacent tissue, the expression levels of the Notch signaling and Notch-1 receptor ligand Jagged-1 in the cancer tissue were significantly lower, suggesting that there is a negative relevance of Notch-1 and Jagged-1 with the development of renal cell carcinoma.3. Compared with the renal cell carcinoma adjacent tissue, the expression levels of Notch-2, another receptor of Notch signaling pathway, and its ligand Jagged-2 in the cancer tissue were significantly higher, suggesting that there is a positive relevance of Notch-1 and Jagged-1 with the development of renal cell carcinoma.